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Literature summary for 3.1.3.4 extracted from

  • Karanasios, E.; Han, G.S.; Xu, Z.; Carman, G.M.; Siniossoglou, S.
    A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase (2010), Proc. Natl. Acad. Sci. USA, 107, 17539-17544.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information dephosphorylation of lipin Pah1p by the Nem1p-Spo7p complex enables the amphipathic helix to anchor Pah1p onto the nuclear/endoplasmic reticulum membrane allowing the production of diacylglycerol for lipid biosynthesis Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
phosphatidic acid wild type enzyme, pH and temperature not specified in the publication Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane recruitment of the yeast lipin (Pah1p) is regulated by PA levels onto the nuclear/endoplasmic reticulum membrane. Recruitment requires the transmembrane protein phosphatase complex Nem1p-Spo7p Saccharomyces cerevisiae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
PtA fusion affinity purification Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidic acid + H2O
-
Saccharomyces cerevisiae 1,2-diacyl-sn-glycerol + phosphate
-
?

Synonyms

Synonyms Comment Organism
lipin
-
Saccharomyces cerevisiae
PA phosphatase
-
Saccharomyces cerevisiae
Pah1p
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function lipins are essential regulators of fat metabolism, adipogenesis, and organelle biogenesis Saccharomyces cerevisiae