Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency, food deprivation, and obesity, often independent of changes in steady-state mRNA levels. Fasting induces expression of Lpin1 and Lpin2 in wild-type and PGC-1alpha-deficient mice | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression analysis of lipin-1 and lipin-2, recombinant expression of lipin-2 in Hep-G2 cells using an adenoviral vector | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | livers of fld mice lacking lipin 1 exhibit normal PAP-1 activity and a 20fold increase in triglyceride levels, while the hepatic content of phosphatidate is markedly diminished in fld mice. Other phospholipids derived from phosphatidate, phosphatidylglycerol and cardiolipin, are also depleted. Hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency via a posttranscriptional mechanism | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes | Mus musculus | 5829 | - |
endoplasmic reticulum membrane | lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes | Mus musculus | 5789 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3-sn-phosphatidate + H2O | Mus musculus | lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme | a 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
additional information | Mus musculus | lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
genes Lpin1 and Lpin2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | serine 106 residue of lipin-1 and lipin-2 is a major site of phosphorylation | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | lipin-1 and high activity of lipin-2 | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3-sn-phosphatidate + H2O | - |
Mus musculus | a 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
a 3-sn-phosphatidate + H2O | lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme | Mus musculus | a 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
additional information | lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipin-1 | - |
Mus musculus |
lipin-2 | - |
Mus musculus |
More | the enzyme is a member of the lipin family | Mus musculus |
PAP-1 | - |
Mus musculus |
phosphatidate phosphohydrolase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |