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Literature summary for 3.1.3.4 extracted from

  • Zhang, Y.; Yang, Z.; Huang, X.; Peng, J.; Fei, X.; Gu, S.; Xie, Y.; Ji, C.; Mao, Y.
    Cloning, expression, and characterization of a thermostable PAP2L2, a new member of the type-2 phosphatidic acid phosphatase family from Geobacillus toebii T-85 (2008), Biosci. Biotechnol. Biochem., 72, 3134-3141.
    View publication on PubMed
Search for more literature for 3.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
genomic library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His6-tagged PAP2L2 in Escherichia coli Parageobacillus toebii

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by N-ethylmaleimide Parageobacillus toebii
SDS 1%, strong inhibition Parageobacillus toebii
vanadate 10 mM, strong inhibition Parageobacillus toebii
Zn2+ strong inhibition Parageobacillus toebii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of the recombinant His6-tagged PAP2L2 Parageobacillus toebii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane six-transmembrane topology Parageobacillus toebii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for Mg2+ Parageobacillus toebii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3-sn-phosphatidate + H2O Parageobacillus toebii
-
 a 1,2-diacyl-sn-glycerol + phosphate
-
 ?
a 3-sn-phosphatidate + H2O Parageobacillus toebii T-85
-
 a 1,2-diacyl-sn-glycerol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Parageobacillus toebii A5HKK6
-
-
Parageobacillus toebii T-85 A5HKK6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged PAP2L2 18.4fold from Escherichia coli by solubilization using 0.3% v/v Triton X-100, and nickel affinity chromatography Parageobacillus toebii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.3
-
 purified recombinant His6-tagged PAP2L2 Parageobacillus toebii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 3-sn-phosphatidate + H2O
-
 Parageobacillus toebii a 1,2-diacyl-sn-glycerol + phosphate
-
 ?
a 3-sn-phosphatidate + H2O
-
 Parageobacillus toebii T-85 a 1,2-diacyl-sn-glycerol + phosphate
-
 ?
diacylglycerol diphosphate + H2O preferred substrate Parageobacillus toebii ? + phosphate
-
 ?
diacylglycerol diphosphate + H2O preferred substrate Parageobacillus toebii T-85 ? + phosphate
-
 ?
lysophosphatidic acid + H2O
-
 Parageobacillus toebii ? + phosphate
-
 ?
lysophosphatidic acid + H2O
-
 Parageobacillus toebii T-85 ? + phosphate
-
 ?
additional information recombinant PAP2L2 shows a broad substrate specificity Parageobacillus toebii ?
-
 ?
additional information recombinant PAP2L2 shows a broad substrate specificity Parageobacillus toebii T-85 ?
-
 ?
phosphatidic acid + H2O preferred substrate Parageobacillus toebii 1,2-sn-diacylglycerol + phosphate
-
 ?
phosphatidic acid + H2O preferred substrate Parageobacillus toebii T-85 1,2-sn-diacylglycerol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the type 2 phosphatidic acid phosphatase superfamily Parageobacillus toebii
PAP2-like protein
-
 Parageobacillus toebii
PAP2L2
-
 Parageobacillus toebii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Parageobacillus toebii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, 50% remaining activity Parageobacillus toebii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Parageobacillus toebii