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Literature summary for 3.1.3.4 extracted from

  • Han, G.S.; Siniossoglou, S.; Carman, G.M.
    The cellular functions of the yeast lipin homolog PAH1p are dependent on its phosphatidate phosphatase activity (2007), J. Biol. Chem., 282, 37026-37035.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
subcloning and expression of wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D398E site-directed mutagenesis, the mutant is almost inactive, the activity is independent of the substrate concentration, the mutant shows aberrant regulation of lipid composition Saccharomyces cerevisiae
D400E site-directed mutagenesis, the mutant is almost inactive, the activity is independent of the substrate concentration, the mutant shows aberrant regulation of lipid composition Saccharomyces cerevisiae
G80R site-directed mutagenesis, the mutant is almost inactive, the activity is independent of the substrate concentration, the mutant shows aberrant regulation of lipid composition Saccharomyces cerevisiae
additional information a pah1DELTA deletion mutant shows a phenotype of temperature sensitivity, respiratory deficiency, nuclear/endoplasmic reticulum membrane expansion, derepression of INO1 expression, and alterations in lipid composition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information kinetic model Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae the enzyme plays a major role in the synthesis of triacylglycerols and phospholipids in Saccharomyces cerevisiae, the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology, role of PAH1-encoded PAP1 in lipid synthesis, pathway, overview ?
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme plays a major role in the synthesis of triacylglycerols and phospholipids in Saccharomyces cerevisiae, the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology, role of PAH1-encoded PAP1 in lipid synthesis, pathway, overview Saccharomyces cerevisiae ?
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?
additional information PAH1-encoded Mg2+-dependent PAP1 catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol and phosphate, PAP1 contains the catalytic motif DIDGT at residues 398–402 and a conserved Gly80 residue Saccharomyces cerevisiae ?
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?

Subunits

Subunits Comment Organism
More PAP1 contains the catalytic motif DIDGT at residues 398–402 and a conserved Gly80 residue Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
3-sn-phosphatidate phosphohydrolase
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Saccharomyces cerevisiae
PAP1
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Saccharomyces cerevisiae