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Literature summary for 3.1.3.4 extracted from

  • Nakamura, Y.; Tsuchiya, M.; Ohta, H.
    Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin (2007), J. Biol. Chem., 282, 29013-29021.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme LPPbeta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree Arabidopsis thaliana
isozyme LPPepsilon1, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation Arabidopsis thaliana
isozyme LPPepsilon2, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic ARabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation Arabidopsis thaliana
isozyme LPPgamma, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of isozyme LPPgamma in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation Arabidopsis thaliana
isozymes LPPdelta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information a lppepsilon1lppepsilon2 double knock-out mutant shows no significant changes in lipid composition Arabidopsis thaliana
additional information a lppgamma homozygous mutant is isolated only under ectopic overexpression of LPPgamma, suggesting that loss of LPPgamma may cause lethal effect on plant viability Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ about 75% inhibition at 20 mM, isozyme LPPgamma; about 95% inhibition at 20 mM, isozyme LPPepsilon1 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the homolog of cyanobacterial LPP, isozyme LPPepsilon1, is localized to chloroplasts and contains a plastidic transit peptide Arabidopsis thaliana 9507
-
chloroplast the homolog of cyanobacterial LPP, isozyme LPPepsilon2, is localized to chloroplasts and contains a plastidic transit peptide Arabidopsis thaliana 9507
-
chloroplast the homolog of cyanobacterial LPP, isozyme LPPgamma, is localized to chloroplasts and contains a plastidic transit peptide Arabidopsis thaliana 9507
-
additional information isozyme LPPbeta contains no plastidic transit peptide Arabidopsis thaliana
-
-
additional information isozyme LPPdelta contains no plastidic transit peptide Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 50% inhibition at 10-20 mM, isozyme LPPepsilon2 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23371
-
x * 23371, isozyme LPPdelta, sequence calculation Arabidopsis thaliana
25736
-
x * 25736, isozyme LPPgamma, sequence calculation Arabidopsis thaliana
30578
-
x * 30578, isozyme LPPepsilon1, sequence calculation Arabidopsis thaliana
31512
-
x * 31512, isozyme LPPepsilon2, sequence calculation Arabidopsis thaliana
46151
-
x * 46151, isozyme LPPdelta, sequence calculation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidic acid + H2O Arabidopsis thaliana
-
1,2-sn-diacylglycerol + phosphate
-
?
phosphatidic acid + H2O Arabidopsis thaliana the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes 1,2-sn-diacylglycerol + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
This UniProt-ID has been deleted; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
Arabidopsis thaliana Q0WUC2 isozyme LPPdelta; ecotype Columbia-0
-
Arabidopsis thaliana Q6NLA5 isozyme LPPgamma; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
Arabidopsis thaliana Q6NQL6 isozyme LPPepsilon2; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
Arabidopsis thaliana Q9SUW4 isozyme LPPbeta; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-

Purification (Commentary)

Purification (Comment) Organism
chloroplast preparation from native and transgenic plants Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
shoot
-
Arabidopsis thaliana
-
shoot high expression of isozyme LPPgamma Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidic acid + H2O
-
Arabidopsis thaliana 1,2-sn-diacylglycerol + phosphate
-
?
phosphatidic acid + H2O the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes Arabidopsis thaliana 1,2-sn-diacylglycerol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 23371, isozyme LPPdelta, sequence calculation Arabidopsis thaliana
? x * 25736, isozyme LPPgamma, sequence calculation Arabidopsis thaliana
? x * 30578, isozyme LPPepsilon1, sequence calculation Arabidopsis thaliana
? x * 31512, isozyme LPPepsilon2, sequence calculation Arabidopsis thaliana
? x * 46151, isozyme LPPdelta, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
lipid phosphate phosphatase
-
Arabidopsis thaliana
LPP
-
Arabidopsis thaliana
More plastidic PAPs in Arabidopsis belong to a distinct lipid phosphate phosphatase LPP subfamily with prokaryotic origin Arabidopsis thaliana
PAP
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 isozyme LPPgamma, broad optimum Arabidopsis thaliana
7
-
isozyme LPPepsilon1 Arabidopsis thaliana
7
-
isozyme LPPepsilon2 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5 10 isozyme LPPepsilon1 Arabidopsis thaliana
5 10 isozyme LPPepsilon2 Arabidopsis thaliana
5 10 isozyme LPPgamma Arabidopsis thaliana