Any feedback?
Literature summary for 3.1.3.4 extracted from
- Pulmonary phosphatidic acid phosphatase. Properties of membrane-bound phosphatidate-dependent phosphatidic acid phosphatase in rat lung (1979), Biochim. Biophys. Acta, 574, 212-225.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1.0-2.0 mM, complete loss of cytosolic activity, reduced microsomal activity | Rattus norvegicus |  |
| Mg2+ | above 5 mM | Rattus norvegicus | |
| phosphatidylcholine | slight inhibition of cytosolic activity | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0054 | - |
phosphatidic acid | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | below 5 mM, slight stimulation of cytosolic activity, inhibition at higher concentrations | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, activity in cytosolic fraction is stable for at least two months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung | Rattus norvegicus | ? | - |
? | |
| phosphatidic acid + H2O | - |
Rattus norvegicus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | membrane-bound phosphatidic acid, microsomal enzyme | Rattus norvegicus |
| 7.4 | 8 | membrane-bound phosphatidic acid, cytosolic enzyme | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
