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Literature summary for 3.1.3.37 extracted from

  • Seuter, A.; Busch, M.; Hain, R.
    Overexpression of the potential herbicide target sedoheptulose-1,7-bisphosphatase from Spinacia oleracea in transgenic tobacco (2002), Mol. Breed., 9, 53-61.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation Spinacia oleracea
sedoheptulose 1,7-bisphosphate activation Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Spinacia oleracea
Mg2+ required, optimal at 15 mM Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence Spinacia oleracea
38000
-
x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
overexpression in Nicotiana tabacum
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification processing of 67 amino acids transit peptide Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
substrate fructose 1,6-bisphosphate, pH 8.3, 22°C Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructose 1,6-bisphosphate + H2O about 20-25% of the activity obtained with seduheptulose 1,7-bisphosphate Spinacia oleracea phosphate + ?
-
?
sedoheptulose 1,7-bisphosphate + H2O
-
Spinacia oleracea sedoheptulose 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Spinacia oleracea