Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activation | Spinacia oleracea | |
sedoheptulose 1,7-bisphosphate | activation | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Spinacia oleracea | |
Mg2+ | required, optimal at 15 mM | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence | Spinacia oleracea |
38000 | - |
x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
overexpression in Nicotiana tabacum | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | processing of 67 amino acids transit peptide | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
substrate fructose 1,6-bisphosphate, pH 8.3, 22°C | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose 1,6-bisphosphate + H2O | about 20-25% of the activity obtained with seduheptulose 1,7-bisphosphate | Spinacia oleracea | phosphate + ? | - |
? | |
sedoheptulose 1,7-bisphosphate + H2O | - |
Spinacia oleracea | sedoheptulose 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, enzyme plus His6-tag plus transit peptide, x * 35000, mature enzyme, deduced from gene sequence | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Spinacia oleracea |