Literature summary for 3.1.3.36 extracted from

Mills, S.J.; Silvander, C.; Cozier, G.; Tresaugues, L.; Nordlund, P.; Potter, B.V.
Crystal Structures of type-II inositol polyphosphate 5-phosphatase INPP5B with synthetic inositol polyphosphate surrogates reveal new mechanistic insights for the inositol 5-phosphatase family (2016), Biochemistry, 55, 1384-1397 .

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with biphenyl 3,3',4,4',5,5'-hexakisphosphate, to 2.89 A resolution. One inhibitor pole locates in the phospholipid headgroup binding site and the second solvent-exposed ring binds to the His-Tag of another INPP5B molecule, while a molecule of inorganic phosphate is also present in the active site. In complex with benzene 1,2,4,5-tetrakisphosphate, to 2.9 A resolution. Conserved residues among the 5-phosphatase family mediate interactions with the inhibitors similar to those with the polar groups present in positions 1, 4, 5, and 6 on the inositol ring of the substrate. 5-Phosphatase specificity most likely resides in the variable zone located close to the 2- and 3-positions of the inositol ring	Homo sapiens

Crystallization (Comment)

Organism

in complex with biphenyl 3,3',4,4',5,5'-hexakisphosphate, to 2.89 A resolution. One inhibitor pole locates in the phospholipid headgroup binding site and the second solvent-exposed ring binds to the His-Tag of another INPP5B molecule, while a molecule of inorganic phosphate is also present in the active site. In complex with benzene 1,2,4,5-tetrakisphosphate, to 2.9 A resolution. Conserved residues among the 5-phosphatase family mediate interactions with the inhibitors similar to those with the polar groups present in positions 1, 4, 5, and 6 on the inositol ring of the substrate. 5-Phosphatase specificity most likely resides in the variable zone located close to the 2- and 3-positions of the inositol ring

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
benzene 1,2,3-trisphosphate	-	Homo sapiens
benzene 1,2,4,5-tetrakisphosphate	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P32019	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O	-	Homo sapiens	1D-myo-inositol 1,3,4-trisphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
INPP5B	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0063	-	pH 7.5, 30°C	Homo sapiens	benzene 1,2,4,5-tetrakisphosphate
0.0335	-	pH 7.5, 30°C	Homo sapiens	benzene 1,2,3-trisphosphate