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Literature summary for 3.1.3.36 extracted from

  • Hung, C.S.; Lin, Y.L.; Wu, C.I.; Huang, C.J.; Ting, L.P.
    Suppression of hepatitis B viral gene expression by phosphoinositide 5-phosphatase SKIP (2009), Cell. Microbiol., 11, 37-50.
    View publication on PubMed
Search for more literature for 3.1.3.36

Application

Application Comment Organism
molecular biology SKIP is translocated from endoplasmic reticulum into nucleus through its interaction with hepatitis B virus core protein and suppresses HBV gene expression via a novel suppression domain Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-fusion protein and as a Flag-tagged fusion protein in human hepatoma HuH-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D192A to assess the role of catalytic activity of SKIP on its suppressive effect, a phosphatase-negative mutant D192A is generated: 5-phosphatase activity is not required for the suppressive effect Homo sapiens
additional information hepatitis B virus core protein interacts with SKIP, both in vivo and in vitro. The minimal sequence required for interaction is the amino acid region from 116 to 149 for the core protein and the SKIP carboxyl homology (SKICH) domain for SKIP. When hepatitis B virus replicates in HuH-7 cells, overexpressed SKIP localizes to nucleus in addition to endoplasmic reticulum and suppresses heptitis B virus gene expression and replication. SKIP loses its nuclear localization and suppressive effect during replication of a core-negative HBV mutant Homo sapiens
additional information in hepatitis B virus infected HuH-7 cells, HBV gene expression is enhanced significantly when endogenous SKIP expression is knocked down by a SKIP-specific siRNA Homo sapiens
additional information mutation analysis show that the suppression domain of SKIP localises to amino acids 199-226 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum SKIP localizes to the ER in HuH-7 cells Homo sapiens 5783
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nucleus in HuH-7 cells overexpressing SKIP and infected with hepatitis B virus (HBV), SKIP localizes to nucleus in addition to endoplasmic reticulum and suppresses HBV gene expression and replication. SKIP loses its nuclear localization and suppressive effect during replication of a core-negative HBV mutant Homo sapiens 5634
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Synonyms

Synonyms Comment Organism
Phosphoinositide 5-phosphatase
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 Homo sapiens
skeletal muscle and kidney enriched inositol phosphatase
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 Homo sapiens
SKIP
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 Homo sapiens