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Literature summary for 3.1.3.36 extracted from

  • Zhong, R.; Ye, Z.H.
    Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana (2004), Plant Cell Physiol., 45, 1720-1728.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene 5PTase14, DNA and amino acid sequence determination and analysis, genetic organization, expression of His-tagged full length enzyme in yeast Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, maximal activity at 3 mM Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Arabidopsis thaliana
-
1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SKB7 gene 5PTase14
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from yeast by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf very low expression of 5PTase14 Arabidopsis thaliana
-
additional information expression pattern Arabidopsis thaliana
-
root low expression of 5PTase14 Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
shoot tip high expression of 5PTase14 Arabidopsis thaliana
-
stem very low expression of 5PTase14 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
Arabidopsis thaliana 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
Arabidopsis thaliana 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O best substrate Arabidopsis thaliana 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
?
1D-myo-inositol 1,4,5-trisphosphate + H2O
-
Arabidopsis thaliana 1D-myo-inositol 1,4-bisphosphate + phosphate
-
?
additional information substrate specificity, no activity with 1D-myo-inositol 1,3,4,5-tetrakisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate, 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1-phosphatidyl-1D-myo-inositol 4-phosphate, and 1-phosphatidyl-1D-myo-inositol 3-phosphate Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
More the enzyme contains a WD-repeat domain and a 5PTase catalytic domain, domain organization Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5PTase
-
Arabidopsis thaliana
inositol polyphosphate 5-phosphatase
-
Arabidopsis thaliana
More cf. EC 3.1.3.56, inositol polyphosphate 5-phosphatase Arabidopsis thaliana
type II inositol polyphosphate 5-phosphatase
-
Arabidopsis thaliana