Cloned (Comment) | Organism |
---|---|
gene 5PTase14, DNA and amino acid sequence determination and analysis, genetic organization, expression of His-tagged full length enzyme in yeast | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, maximal activity at 3 mM | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | Arabidopsis thaliana | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SKB7 | gene 5PTase14 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from yeast by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | very low expression of 5PTase14 | Arabidopsis thaliana | - |
additional information | expression pattern | Arabidopsis thaliana | - |
root | low expression of 5PTase14 | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
shoot tip | high expression of 5PTase14 | Arabidopsis thaliana | - |
stem | very low expression of 5PTase14 | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O | - |
Arabidopsis thaliana | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Arabidopsis thaliana | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | best substrate | Arabidopsis thaliana | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? | |
1D-myo-inositol 1,4,5-trisphosphate + H2O | - |
Arabidopsis thaliana | 1D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
additional information | substrate specificity, no activity with 1D-myo-inositol 1,3,4,5-tetrakisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate, 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1-phosphatidyl-1D-myo-inositol 4-phosphate, and 1-phosphatidyl-1D-myo-inositol 3-phosphate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains a WD-repeat domain and a 5PTase catalytic domain, domain organization | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
5PTase | - |
Arabidopsis thaliana |
inositol polyphosphate 5-phosphatase | - |
Arabidopsis thaliana |
More | cf. EC 3.1.3.56, inositol polyphosphate 5-phosphatase | Arabidopsis thaliana |
type II inositol polyphosphate 5-phosphatase | - |
Arabidopsis thaliana |