The enzymatic cleavage of phosphate termini from polynucleotides

Becker, A.; Hurwitz, J.; J. Biol. Chem. 242, 936-950 (1967)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

p-hydroxymercuribenzoate

in absence of sulfhydryl compounds

Escherichia coli

Metals/Ions

Commentary

Organism

Structure

CoCl2

divalent metal required, 30% the rate observed with MgCl2

Escherichia coli

MgCl2

divalent metal required

Escherichia coli

MnCl2

divalent metal required, 60% the rate observed with MgCl2

Escherichia coli

additional information

no cleavage in absence of divalent metal ion

Escherichia coli

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

5'-phosphyl-terminated DNA + H2O

Escherichia coli

-

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

5'-phosphyl-terminated DNA + H2O

-

81080

Escherichia coli

?

-

-

-

-

5'-phosphyl-terminated DNA + H2O

-

81080

Escherichia coli

?

-

-

-

?

Inhibitors

Commentary

Organism

Structure

p-hydroxymercuribenzoate

in absence of sulfhydryl compounds

Escherichia coli

Metals/Ions

Commentary

Organism

Structure

CoCl2

divalent metal required, 30% the rate observed with MgCl2

Escherichia coli

MgCl2

divalent metal required

Escherichia coli

MnCl2

divalent metal required, 60% the rate observed with MgCl2

Escherichia coli

additional information

no cleavage in absence of divalent metal ion

Escherichia coli

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

5'-phosphyl-terminated DNA + H2O

Escherichia coli

-

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

5'-phosphyl-terminated DNA + H2O

-

81080

Escherichia coli

?

-

-

-

-

5'-phosphyl-terminated DNA + H2O

-

81080

Escherichia coli

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

81080

Becker

The enzymatic cleavage of phos ...

Escherichia coli

J. Biol. Chem.

242

936-950

1967

-

-

-

-

-

-

1

-

-

4

-

1

-

1

-

-

1

-

-

-

1

-

2

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

4

-

1

-

-

-

1

-

-

1

-

2

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-