BRENDA - Enzyme Database
show all sequences of 3.1.3.33

The enzymatic cleavage of phosphate termini from polynucleotides

Becker, A.; Hurwitz, J.; J. Biol. Chem. 242, 936-950 (1967)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
in absence of sulfhydryl compounds
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CoCl2
divalent metal required, 30% the rate observed with MgCl2
Escherichia coli
MgCl2
divalent metal required
Escherichia coli
MnCl2
divalent metal required, 60% the rate observed with MgCl2
Escherichia coli
additional information
no cleavage in absence of divalent metal ion
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5'-phosphyl-terminated DNA + H2O
Escherichia coli
-
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
B, induced by T4am82
-
Purification (Commentary)
Purification (Commentary)
Organism
induced by T4am82
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2460
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5'-phosphyl-terminated DNA + H2O
-
81080
Escherichia coli
?
-
-
-
-
5'-phosphyl-terminated DNA + H2O
-
81080
Escherichia coli
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
6.8
50% of maximal activity at pH 5.5 and pH 6.8
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
in absence of sulfhydryl compounds
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CoCl2
divalent metal required, 30% the rate observed with MgCl2
Escherichia coli
MgCl2
divalent metal required
Escherichia coli
MnCl2
divalent metal required, 60% the rate observed with MgCl2
Escherichia coli
additional information
no cleavage in absence of divalent metal ion
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5'-phosphyl-terminated DNA + H2O
Escherichia coli
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
induced by T4am82
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2460
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5'-phosphyl-terminated DNA + H2O
-
81080
Escherichia coli
?
-
-
-
-
5'-phosphyl-terminated DNA + H2O
-
81080
Escherichia coli
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
6.8
50% of maximal activity at pH 5.5 and pH 6.8
Escherichia coli
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
81080
Becker
The enzymatic cleavage of phos ...
Escherichia coli
J. Biol. Chem.
242
936-950
1967
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