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Literature summary for 3.1.3.32 extracted from
- Further purification and characterization of the DNA 3-phosphatase from rat-liver chromatin which is also a polynucleotide 5-hydroxyl kinase (1988), Eur. J. Biochem., 171, 59-66.
General Stability
| General Stability | Organism |
|---|---|
| ATP protects against thermal denaturation and trypsin digestion | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | no effect on 3'-phosphatase, inhibition of 5'-hydroxyl kinase | Rattus norvegicus |  |
| EDTA | - |
Rattus norvegicus | |
| PO43- | 5'-hydroxyl kinase, little effect on 3'-phosphatase | Rattus norvegicus | |
| SO42- | 5'-hydroxyl kinase, little effect on 3'-phosphatase | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0039 | - |
DNA with 5'-hydroxyl ends | - |
Rattus norvegicus | |
| 0.069 | - |
ATP | - |
Rattus norvegicus | |
| 0.548 | - |
3'-phosphopolynucleotide | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromatin | - |
Rattus norvegicus | 785 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates | Rattus norvegicus | |
| Mn2+ | stimulates | Rattus norvegicus | |
| Zn2+ | stimulates less efficiently than Mg2+, Mn2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Rattus norvegicus |
| 79000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
enzyme also is polynucleotide 5'-hydroxyl kinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme also is polynucleotide 5'-hydroxyl kinase | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate | also 5-hydroxylkinase activity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphopolynucleotide + H2O | - |
Rattus norvegicus | polynucleotide + phosphate | - |
? | |
| DNA with 3'-phosphate ends + H2O | - |
Rattus norvegicus | DNA + phosphate | - |
? | |
| DNA with 5'-hydroxyl ends + ATP | - |
Rattus norvegicus | DNA with 5'-phosphate ends + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
5'-hydroxyl kinase activity inactivated much faster than 3'-phosphatase activity, ATP protects against thermal denaturation | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
5'-hydroxyl kinase activity | Rattus norvegicus |
| 6 | - |
3'-phosphatase activity, 2 optima: pH 6.0 and pH 7.5 | Rattus norvegicus |
| 7.5 | - |
3'-phosphatase activity, 2 optima: pH 6.0 and pH 7.5 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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