Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.32 extracted from

  • Das, U.; Shuman, S.
    Mechanism of RNA 2,3-cyclic phosphate end healing by T4 polynucleotide kinase-phosphatase (2013), Nucleic Acids Res., 41, 355-365.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
D167N the mutant converts a RNA 2',3'-cyclic phosphate end to RNA 3'-phosphate Tequatrovirus T4
S211A the mutant transiently accumulates phosphor-RNA during 2',3'-cyclic phosphate removal Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phospho-5'-hydroxy-RNA + H2O Tequatrovirus T4
-
 ?
-
 ?
a RNA 2',3'-cyclic phosphate end + H2O Tequatrovirus T4 the enzyme also has RNA 2'-phosphatase activity a RNA 3'-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-agarose column chromatography Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phospho-5'-hydroxy-RNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
a RNA 2',3'-cyclic phosphate end + H2O the enzyme also has RNA 2'-phosphatase activity Tequatrovirus T4 a RNA 3'-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PNKP
-
 Tequatrovirus T4
polynucleotide kinase-phosphatase
-
 Tequatrovirus T4