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Literature summary for 3.1.3.32 extracted from

  • Tahbaz, N.; Subedi, S.; Weinfeld, M.
    Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair (2012), Nucleic Acids Res., 40, 3484-3495.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.32

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-or C-terminally His-tagged enzyme in A-549 cells, expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information kinase negative PNKP is generated by site-directed mutagenesis using RNAi-resistant PNKP cDNA. Phosphatase negative PNKP is generated by site-directed mutagenesis using RNAi-resistant PNKP cDNA, enzyme-deficient A-549 cells are constructed by expression of shRNA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the enzyme contains a functional mitochondrial-targeting signal, consisting of amino acids 432-441 (ARYVQCARAA), close to its C-terminus Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphopolynucleotide + H2O Homo sapiens
-
 polynucleotide + phosphate
-
 ?
additional information Homo sapiens the enzyme acts on mitochondrial DNA and interacts with the mitochondrial proteins, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphopolynucleotide + H2O
-
 Homo sapiens polynucleotide + phosphate
-
 ?
additional information the enzyme acts on mitochondrial DNA and interacts with the mitochondrial proteins, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
polynucleotide kinase/phosphatase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the mitochondrial enzyme polynucleotide kinase/phosphatase is required in mitochondrial DNA repair, overview Homo sapiens