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Literature summary for 3.1.3.32 extracted from

  • Mani, R.S.; Yu, Y.; Fang, S.; Lu, M.; Fanta, M.; Zolner, A.E.; Tahbaz, N.; Ramsden, D.A.; Litchfield, D.W.; Lees-Miller, S.P.; Weinfeld, M.
    Dual modes of interaction between XRCC4 and polynucleotide kinase/phosphatase: implications for nonhomologous end joining (2010), J. Biol. Chem., 285, 37619-37629.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene PfPNKP, DNA and amino acid sequence determination and analysis, expression of GST-PfPNKP in Escherichia coli strain Rosetta (DE3) pLysS Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Plasmodium falciparum polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. Recombinant GFP-tagged PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3-single-stranded overhang, but not double-stranded substrates that mimicked single-strand breaks ?
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. Recombinant GFP-tagged PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3-single-stranded overhang, but not double-stranded substrates that mimicked single-strand breaks Plasmodium falciparum ?
-
 ?

Synonyms

Synonyms Comment Organism
PNKP
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 Plasmodium falciparum
polynucleotide kinase/phosphatase
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Plasmodium falciparum

General Information

General Information Comment Organism
physiological function polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. It is a DNA repair enzyme involved in the processing of strand break termini, which permits subsequent repair proteins to replace missing nucleotides and rejoin broken strands. PfPNKP may not be involved in single-strand break repair, since alternative terminal processing mechanisms can substitute for PfPNKP, and that PfPNKP DNA repair actions may be confined to overhanging termini of double-strand breaks Plasmodium falciparum