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Literature summary for 3.1.3.32 extracted from

  • Zhu, H.; Smith, P.; Wang, L.K.; Shuman, S.
    Structure-function analysis of the 3' phosphatase component of T4 polynucleotide kinase/phosphatase (2007), Virology, 366, 126-136.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Tequatrovirus T4

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 100 mM sodium cacodylate (pH 6.6), 12% PEG-8000, 0.2 M ammonium sulfate, 20 mM urea, and 5 mM dithiothreitol, at 23°C Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
D187A less than 1% activity of the wild type enzyme Tequatrovirus T4
D187E less than 1% activity of the wild type enzyme Tequatrovirus T4
D187N less than 1% activity of the wild type enzyme Tequatrovirus T4
D277A less than 1% activity of the wild type enzyme Tequatrovirus T4
D277E retains 93% activity of the wild type enzyme Tequatrovirus T4
D277N less than 1% activity of the wild type enzyme Tequatrovirus T4
E195A/Y205A retains 22% activity of the wild type enzyme Tequatrovirus T4
E219A retains 51% activity of the wild type enzyme Tequatrovirus T4
E233A retains 45% activity of the wild type enzyme Tequatrovirus T4
E292A/W294A retains 3% activity of the wild type enzyme Tequatrovirus T4
G212A retains 37% activity of the wild type enzyme Tequatrovirus T4
K198A retains 82% activity of the wild type enzyme Tequatrovirus T4
K253A retains 95% activity of the wild type enzyme Tequatrovirus T4
K258A less than 1% activity of the wild type enzyme Tequatrovirus T4
K258Q less than 1% activity of the wild type enzyme Tequatrovirus T4
K258R less than 1% activity of the wild type enzyme Tequatrovirus T4
M199A/Y200A retains 19% activity of the wild type enzyme Tequatrovirus T4
N190A/M192A retains 18% activity of the wild type enzyme Tequatrovirus T4
R229A retains 94% activity of the wild type enzyme Tequatrovirus T4
R287A/Q295A retains 2% activity of the wild type enzyme Tequatrovirus T4
S211A less than 1% activity of the wild type enzyme Tequatrovirus T4
S211T shows 130% activity of the wild type enzyme Tequatrovirus T4
S298A/D300A retains 27% activity of the wild type enzyme Tequatrovirus T4
T222A retains 94% activity of the wild type enzyme Tequatrovirus T4
T251A shows 120% activity of the wild type enzyme Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid-agarose chromatography Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phospho-5'-hydroxy-DNA
-
 Tequatrovirus T4 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Tequatrovirus T4

Synonyms

Synonyms Comment Organism
3' phosphatase
-
 Tequatrovirus T4
PNKP
-
 Tequatrovirus T4
T4 polynucleotide kinase/phosphatase
-
 Tequatrovirus T4