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Literature summary for 3.1.3.32 extracted from
- Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40 (2004), J. Biol. Chem., 279, 26358-26369.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D169A | half of wild type 5-kinase activity, almost complete loss of 3-phosphatase activity | Kostyavirus CJW1 |
| D175A | reduced 5-kinase activity, no 3-phosphatase activity | Omegavirus omega |
| K15A | no 5-kinase activity, reduced 3-phosphates activity | Kostyavirus CJW1 |
| K16A | retains 5-kinase activity, reduced 3-phosphatase activity | Omegavirus omega |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, order of decreasing preference: Co2+, Mg2+, Mn2+ | Kostyavirus CJW1 |  |
| Co2+ | divalent metal ion required, fulfilled by Co2+, Mg2+, Mn2+ at 10 mM | Omegavirus omega | |
| Mg2+ | divalent cation required, order of decreasing preference: Co2+, Mg2+, Mn2+ | Kostyavirus CJW1 | |
| Mg2+ | divalent metal ion required, fulfilled by Co2+, Mg2+, Mn2+ at 10 mM | Omegavirus omega | |
| Mn2+ | divalent cation required, order of decreasing preference: Co2+, Mg2+, Mn2+ | Kostyavirus CJW1 | |
| Mn2+ | divalent metal ion required, fulfilled by Co2+, Mg2+, Mn2+ at 10 mM | Omegavirus omega | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, His10-tagged enzyme, SDS-PAGE | Omegavirus omega |
| 80000 | 160000 | sedimentation analysis | Omegavirus omega |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kostyavirus CJW1 | - |
bifunctional enzyme with proximal 5-kinase and disal 3-phosphatase activity | - |
| Omegavirus omega | - |
bifunctional enzyme with proximal 5-kinase and disal 3-phosphatase activity | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19 | - |
3-phosphatase activity | Omegavirus omega |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTMP + H2O | - |
Omegavirus omega | thymidine + phosphate | - |
? | |
| dTMP + H2O | - |
Kostyavirus CJW1 | thymidine + phosphate | - |
? | |
| phosphorylated DNA ends + H2O | - |
Omegavirus omega | ? | - |
? | |
| phosphorylated DNA ends + H2O | - |
Kostyavirus CJW1 | ? | - |
? | |
| phosphorylated DNA-ends + H2O | - |
Omegavirus omega | DNA-ends + phosphate | - |
r | |
| phosphorylated DNA-ends + H2O | - |
Kostyavirus CJW1 | DNA-ends + phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, His10-tagged enzyme, SDS-PAGE | Omegavirus omega |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNKP | - |
Omegavirus omega |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
phosphorylated DNA ends | - |
Omegavirus omega | |
| 0.0043 | - |
phosphorylated DNA ends | - |
Kostyavirus CJW1 | |
| 0.58 | - |
dTMP | - |
Kostyavirus CJW1 | |
| 0.63 | - |
dTMP | - |
Omegavirus omega | |
| 6.08 | - |
dTMP | - |
Omegavirus omega | |
| 6.08 | - |
dTMP | - |
Kostyavirus CJW1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Omegavirus omega |
| 5.5 | - |
- |
Kostyavirus CJW1 |
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