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Literature summary for 3.1.3.3 extracted from

  • Chiba, Y.; Yoshida, A.; Shimamura, S.; Kameya, M.; Tomita, T.; Nishiyama, M.; Takai, K.
    Discovery and analysis of a novel type of the serine biosynthetic enzyme phosphoserine phosphatase in Thermus thermophilus (2018), FEBS J., 286, 726-736 .
    View publication on PubMed
Search for more literature for 3.1.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ less than 50% activity at 1 mM Thermus thermophilus
Cu2+ less than 50% activity at 1 mM Thermus thermophilus
EDTA about 80% inhibition at 5 mM Thermus thermophilus
Mn2+ less than 50% activity at 1 mM Thermus thermophilus
Zn2+ less than 50% activity at 1 mM Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 O-phospho-L-serine at pH 7.0 and 70°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ may replace Mg2+ (80% activity at 1 mM) Thermus thermophilus
Mg2+ required (100% activity at 1 mM) Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52100
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-D-serine + H2O Thermus thermophilus
-
 D-serine + phosphate
-
 ?
O-phospho-L-serine + H2O Thermus thermophilus
-
 L-serine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 column chromatography, butyl-Toyopearl column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with O-phospho-L-tyrosine, 4-nitrophenyl phosphate, pyridoxal 5'-phosphate, D-glucose 6-phosphate, an D-fructose 6-phosphate Thermus thermophilus ?
-
 ?
O-phospho-D-serine + H2O
-
 Thermus thermophilus D-serine + phosphate
-
 ?
O-phospho-D-serine + H2O highest activity Thermus thermophilus D-serine + phosphate
-
 ?
O-phospho-L-serine + H2O
-
 Thermus thermophilus L-serine + phosphate
-
 ?
O-phospho-L-serine + H2O high activity Thermus thermophilus L-serine + phosphate
-
 ?
O-phospho-L-threonine + H2O lowest activity Thermus thermophilus L-threonine + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 27436, calculated from SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
PSP
-
 Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1506
-
 O-phospho-L-serine at pH 7.0 and 70°C Thermus thermophilus