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Literature summary for 3.1.3.3 extracted from
- Discovery of an intermolecular disulfide bond required for the thermostability of a heterodimeric protein from the thermophile Hydrogenobacter thermophilus (2016), Biosci. Biotechnol. Biochem., 80, 232-240 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells | Hydrogenobacter thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phospho-L-serine + H2O | Hydrogenobacter thermophilus | - |
L-serine + phosphate | - |
? |  |
| O-phospho-L-serine + H2O | Hydrogenobacter thermophilus DSM 6534 | - |
L-serine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenobacter thermophilus | - |
- |
- |
| Hydrogenobacter thermophilus DSM 6534 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, butyl-Toyopearl column chromatography, and MonoQ column chromatography | Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phospho-L-serine + H2O | - |
Hydrogenobacter thermophilus | L-serine + phosphate | - |
? | |
| O-phospho-L-serine + H2O | - |
Hydrogenobacter thermophilus DSM 6534 | L-serine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| iPSP1 | isoform | Hydrogenobacter thermophilus |
| iPSP2 | isoform | Hydrogenobacter thermophilus |
| metal-independent phosphoserine phosphatase | - |
Hydrogenobacter thermophilus |
| pspA | isoform | Hydrogenobacter thermophilus |
| pspB | isoform | Hydrogenobacter thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | the enzyme activity remains stable after 10 min at 70-80°C and drops with further increasing temperature (about 80% activity at 85°C and about 10% activity at 90°C) | Hydrogenobacter thermophilus |
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Release 2023.1 (January 2023)
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