BRENDA - Enzyme Database
show all sequences of 3.1.3.29

Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase

Maliekal, P.; Vertommen, D.; Delpierre, G.; Van Schaftingen, E.; Glycobiology 16, 165-172 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as a fusion protein with a polyHis tag
Homo sapiens
expressed in Escherichia coli as a fusion protein with a polyHis tag
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Homo sapiens
Ca2+
-
Rattus norvegicus
N-acetylneuraminate
-
Homo sapiens
N-acetylneuraminate
-
Rattus norvegicus
vanadate
-
Homo sapiens
vanadate
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
N-acetylneuraminate 9-phosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
0.43
-
N-acetylneuraminate 9-phosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
2.7
-
6-phospho-D-gluconate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
5.9
-
N-acetylglucosamine 6-phosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
19.2
-
D-fructose 1,6-bisphosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
dependent
Homo sapiens
Mg2+
dependent
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, Sephacryl S-200 gel filtration, and UnoQ gel filtration
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.56
-
crude extract, at 37C
Rattus norvegicus
684
-
after 1221fold purification, at 37C
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-phospho-D-gluconate + H2O
-
680033
Homo sapiens
D-gluconate + phosphate
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
-
680033
Homo sapiens
D-fructose 6-phosphate + phosphate
-
-
-
?
additional information
shows no activity towards sorbitol 6-phosphate, 3-phospho-glycerate, phospho-serine, glycerol 3-phosphate, ribulose 5-phosphate, N-acetylmannosamine 6-phosphate, arabinose 5-phosphate, ribose 5-phosphate, glucosamine 6-phosphate, phosphoglycolate, fructose 6-phosphate, glycerol 2-phosphate, glucose 6-phosphate, mannose 6-phosphate, glucose 1-phosphate, phosphoenolpyruvate, and ATP
680033
Homo sapiens
?
-
-
-
-
N-acetylglucosamine 6-phosphate + H2O
-
680033
Homo sapiens
N-acetylglucosamine + phosphate
-
-
-
?
N-acetylneuraminate 9-phosphate + H2O
-
680033
Homo sapiens
N-acetylneuraminate + phosphate
-
-
-
?
N-acetylneuraminate 9-phosphate + H2O
-
680033
Rattus norvegicus
N-acetylneuraminate + phosphate
-
-
-
?
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.012
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
vanadate
0.019
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
vanadate
0.45
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
Ca2+
15
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
N-acetylneuraminate
15
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
N-acetylneuraminate
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as a fusion protein with a polyHis tag
Homo sapiens
expressed in Escherichia coli as a fusion protein with a polyHis tag
Rattus norvegicus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.012
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
vanadate
0.019
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
vanadate
0.45
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
Ca2+
15
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
N-acetylneuraminate
15
-
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
N-acetylneuraminate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Homo sapiens
Ca2+
-
Rattus norvegicus
N-acetylneuraminate
-
Homo sapiens
N-acetylneuraminate
-
Rattus norvegicus
vanadate
-
Homo sapiens
vanadate
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
N-acetylneuraminate 9-phosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
0.43
-
N-acetylneuraminate 9-phosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Rattus norvegicus
2.7
-
6-phospho-D-gluconate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
5.9
-
N-acetylglucosamine 6-phosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
19.2
-
D-fructose 1,6-bisphosphate
in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
dependent
Homo sapiens
Mg2+
dependent
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, Sephacryl S-200 gel filtration, and UnoQ gel filtration
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.56
-
crude extract, at 37C
Rattus norvegicus
684
-
after 1221fold purification, at 37C
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-phospho-D-gluconate + H2O
-
680033
Homo sapiens
D-gluconate + phosphate
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
-
680033
Homo sapiens
D-fructose 6-phosphate + phosphate
-
-
-
?
additional information
shows no activity towards sorbitol 6-phosphate, 3-phospho-glycerate, phospho-serine, glycerol 3-phosphate, ribulose 5-phosphate, N-acetylmannosamine 6-phosphate, arabinose 5-phosphate, ribose 5-phosphate, glucosamine 6-phosphate, phosphoglycolate, fructose 6-phosphate, glycerol 2-phosphate, glucose 6-phosphate, mannose 6-phosphate, glucose 1-phosphate, phosphoenolpyruvate, and ATP
680033
Homo sapiens
?
-
-
-
-
N-acetylglucosamine 6-phosphate + H2O
-
680033
Homo sapiens
N-acetylglucosamine + phosphate
-
-
-
?
N-acetylneuraminate 9-phosphate + H2O
-
680033
Homo sapiens
N-acetylneuraminate + phosphate
-
-
-
?
N-acetylneuraminate 9-phosphate + H2O
-
680033
Rattus norvegicus
N-acetylneuraminate + phosphate
-
-
-
?
Other publictions for EC 3.1.3.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729375
Kim
Design, synthesis, functional ...
Homo sapiens
Bioorg. Med. Chem. Lett.
23
4107-4111
2013
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680033
Maliekal
Identification of the sequence ...
Homo sapiens, Rattus norvegicus
Glycobiology
16
165-172
2006
-
-
2
-
-
-
6
5
-
2
-
-
-
6
-
-
1
-
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
5
6
-
5
-
2
-
-
-
-
-
1
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
81039
Van Rinsum
Subcellular localization and t ...
Rattus norvegicus, Rattus norvegicus Wistar
Biochem. J.
223
323-328
1984
-
-
-
-
-
-
-
1
1
-
-
2
-
166
-
-
-
-
-
10
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
2
-
-
-
-
-
10
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
81040
Ferwerda
Synthesis of N-acetylneuramini ...
Rattus norvegicus
Biochem. J.
216
87-92
1983
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
81038
Jourdian
-
N-Acetylneuraminic (sialic) ac ...
Homo sapiens
Methods Enzymol.
8
205-208
1966
-
-
-
-
-
1
-
1
-
1
-
1
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-