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Literature summary for 3.1.3.27 extracted from

  • Icho, T.; Raetz, C.R.H.
    Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors (1983), J. Bacteriol., 153, 722-730.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.27

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.01 - 0.10 mM Rattus sp.
EGTA 0.01 - 0.10 mM Rattus sp.
phosphatidic acid sodium salt 2 fold stimulation at 3.0 mM Rattus sp.

General Stability

General Stability Organism
sensitive to sulfhydryl reagents and freeze-thaw cycles Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Rattus sp.
Co2+
-
 Rattus sp.
Cu2+
-
 Rattus sp.
Hg2+ complete inhibition at 1 mM Rattus sp.
iodoacetamide
-
 Rattus sp.
ionic and non-ionic detergents most effective is Triton X-100 Rattus sp.
Mg2+
-
 Rattus sp.
Mn2+
-
 Rattus sp.
NaF
-
 Rattus sp.
p-mercuribenzoic acid
-
 Rattus sp.
phosphatidic acid ammonium salt 3.0 mM Rattus sp.
Triton X-100
-
 Rattus sp.
Zn2+
-
 Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 phosphatidylglycerophosphate
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane weak association to the mitochondrial membrane Rattus sp. 31966
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylglycerophosphate + H2O Rattus sp. biosynthesis of phospholipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0017
-
 partially purified Rattus sp.

Storage Stability

Storage Stability Organism
-20°C, stable for several months in 0.25 sucrose, 0.1 mM EDTA, pH 7.4 Rattus sp.
4°C, stable for a number of weeks in 20% glycerol, 0.01 M Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM beta-mercaptoethanol Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylglycerophosphate + H2O
-
 Rattus sp. phosphatidylglycerol + phosphate
-
 ?
phosphatidylglycerophosphate + H2O biosynthesis of phospholipids Rattus sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Rattus sp.