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Literature summary for 3.1.3.26 extracted from

  • Mahajan, A.; Dua, S.
    Nonchemical approach for reducing antinutritional factors in rapeseed (Brassica campestris var. Toria) and characterization of enzyme phytase (1997), J. Agric. Food Chem., 45, 2504-2508.
    View publication on PubMed
Search for more literature for 3.1.3.26

Activating Compound

Activating Compound Comment Organism Structure
EDTA promotes activity Brassica rapa subsp. oleifera

Inhibitors

Inhibitors Comment Organism Structure
arsenate
-
 Brassica rapa subsp. oleifera
Cu2+
-
 Brassica rapa subsp. oleifera
F-
-
 Brassica rapa subsp. oleifera
Fe2+
-
 Brassica rapa subsp. oleifera
molybdate
-
 Brassica rapa subsp. oleifera
phosphate 4.0 mM, 75.5% of phytase 1 activity, 80.2% inhibition of phytase 2 activity Brassica rapa subsp. oleifera
Zn2+
-
 Brassica rapa subsp. oleifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 myo-inositol hexakisphosphate phytase 2 Brassica rapa subsp. oleifera
0.37
-
 myo-inositol hexakisphosphate phytase 1 Brassica rapa subsp. oleifera

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Brassica rapa subsp. oleifera
Mn2+ activates Brassica rapa subsp. oleifera

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. oleifera
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Brassica rapa subsp. oleifera
-
 var. toria
-

Purification (Commentary)

Purification (Comment) Organism
2 phytase fractions: phytase 1 and phytase 2 Brassica rapa subsp. oleifera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Brassica rapa subsp. oleifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Brassica rapa subsp. oleifera myo-inositol pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Brassica rapa subsp. oleifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Brassica rapa subsp. oleifera
70
-
 complete inactivation after 50 min Brassica rapa subsp. oleifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
 phytase 1 and 2 Brassica rapa subsp. oleifera