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Literature summary for 3.1.3.26 extracted from

  • Lolas, G.M.; Markakis, P.
    The phytase of navy beans (Phaseolus aureus) (1977), J. Food Sci., 42, 1094-1097.
No PubMed abstract available

General Stability

General Stability Organism
the enzyme can withstand repeated freezing and thawing without appreciable loss of acticity Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
F- 10 mM, 80% loss of activity Phaseolus vulgaris
iodoacetamide no inhibition up to 10 mM Phaseolus vulgaris
myo-inositol hexakisphosphate above 1-2 mM Phaseolus vulgaris
NaCl 0.1 M, 10% loss of activity, 0.5 M, 50% loss of activity Phaseolus vulgaris
NEM no inhibition up to 10 mM Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
myo-inositol hexakisphosphate
-
Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 35% increase in activity Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
-
Phaseolus vulgaris

Storage Stability

Storage Stability Organism
2°C, 0.01 M Tris-maleate buffer, pH 6.5, stable for at least 6 weeks Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O 9.7fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris adenosine + phosphate
-
?
diphosphate + H2O 735fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris 2 phosphate
-
?
glycerol 2-phosphate + H2O 19fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris glycerol + phosphate
-
?
glycerol 3-phosphate + H2O 15fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris glycerol + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Phaseolus vulgaris myo-inositol pentakisphosphate + phosphate
-
?
phenyl phosphate + H2O 61.7fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris phenol + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Phaseolus vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
-
Phaseolus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, stable Phaseolus vulgaris
65
-
10 min, 55% loss of activity Phaseolus vulgaris
80
-
10 min, complete inactivation Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
-
Phaseolus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 pH 4.5: about 45% of maximal activity, pH 6.0: about 60% of maximal activity Phaseolus vulgaris