General Stability | Organism |
---|---|
the enzyme can withstand repeated freezing and thawing without appreciable loss of acticity | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | 10 mM, 80% loss of activity | Phaseolus vulgaris | |
iodoacetamide | no inhibition up to 10 mM | Phaseolus vulgaris | |
myo-inositol hexakisphosphate | above 1-2 mM | Phaseolus vulgaris | |
NaCl | 0.1 M, 10% loss of activity, 0.5 M, 50% loss of activity | Phaseolus vulgaris | |
NEM | no inhibition up to 10 mM | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
myo-inositol hexakisphosphate | - |
Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 35% increase in activity | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus vulgaris |
Storage Stability | Organism |
---|---|
2°C, 0.01 M Tris-maleate buffer, pH 6.5, stable for at least 6 weeks | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | 9.7fold higher activity than with myo-inositol hexakisphosphate | Phaseolus vulgaris | adenosine + phosphate | - |
? | |
diphosphate + H2O | 735fold higher activity than with myo-inositol hexakisphosphate | Phaseolus vulgaris | 2 phosphate | - |
? | |
glycerol 2-phosphate + H2O | 19fold higher activity than with myo-inositol hexakisphosphate | Phaseolus vulgaris | glycerol + phosphate | - |
? | |
glycerol 3-phosphate + H2O | 15fold higher activity than with myo-inositol hexakisphosphate | Phaseolus vulgaris | glycerol + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Phaseolus vulgaris | myo-inositol pentakisphosphate + phosphate | - |
? | |
phenyl phosphate + H2O | 61.7fold higher activity than with myo-inositol hexakisphosphate | Phaseolus vulgaris | phenol + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Phaseolus vulgaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phaseolus vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, stable | Phaseolus vulgaris |
65 | - |
10 min, 55% loss of activity | Phaseolus vulgaris |
80 | - |
10 min, complete inactivation | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Phaseolus vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6 | pH 4.5: about 45% of maximal activity, pH 6.0: about 60% of maximal activity | Phaseolus vulgaris |