Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | slight activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight | Escherichia coli | |
Cu2+ | strong | Escherichia coli | |
F- | - |
Escherichia coli | |
Mn2+ | slight | Escherichia coli | |
vanadate | - |
Escherichia coli | |
Zn2+ | strong | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
myo-inositol hexakisphosphate | - |
Escherichia coli | |
7.78 | - |
p-nitrophenyl phosphate | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | 2 isoenzymes: P1 and P2 | Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
1 * 42000, SDS-PAGE | Escherichia coli |
43000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | highest activity under anaerobic conditions, during the exponential growth no activity observed | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 ATCC 33965 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8016 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | highest activity under anaerobic conditions, during the exponential growth no activity observed | Escherichia coli | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | myo-inositol pentakisphosphate + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Escherichia coli | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 1 h, no loss of activity | Escherichia coli |
60 | - |
1 h, 76% loss of activity | Escherichia coli |
70 | - |
1 h, complete loss of activity | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
490 | - |
p-nitrophenyl phosphate | - |
Escherichia coli | |
6210 | - |
myo-inositol hexakisphosphate | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
hydrolysis of myo-inositol hexakisphosphate | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5.7 | pH 3.0: about 30% of maximal activity, pH 5.7: about 70% of maximal activity, hydrolysis of myo-inositol hexakisphosphate | Escherichia coli |