Any feedback?
Literature summary for 3.1.3.26 extracted from
- Cloning, recombinant expression and characterization of a new phytase from Penicillium chrysogenum (2015), Microbiol. Res., 170, 205-212 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene phy, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Penicillium griseoroseum strain PG63 protoplasts from vectorpAN-52-1-phy. Transformant T73 contains the phy gene integrated in at least three sites of the genome and shows the highest, 5.1fold increase in phytase activity in comparison to the host strain | Penicillium chrysogenum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme sequence contains a signal peptide and a signal peptide cleavage site | Penicillium chrysogenum | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | Penicillium chrysogenum | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |  |
| myo-inositol hexakisphosphate + H2O | Penicillium chrysogenum CCT 1273 | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
| Penicillium chrysogenum CCT 1273 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme sequence harbors putative N-linked glycosylation sites | Penicillium chrysogenum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium chrysogenum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1350 | - |
native enzyme from Penicillium chrysogenum strain CCT 1273, pH 5.0, 50°C | Penicillium chrysogenum |
| 2860 | - |
recombinant enzyme from Penicillium griseoroseum strain T73, pH 5.0, 50°C | Penicillium chrysogenum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| PHY produced by Penicllium griseoroseum strain T73 retains 75%, 66% and 68% of the initial activity after four weeks of storage at -20°C, 8°C, and 25°C, respectively | Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme catalyzes phosphate release from cereals such as corn, soybean, and wheat | Penicillium chrysogenum | ? | - |
? | |
| additional information | the enzyme catalyzes phosphate release from cereals such as corn, soybean, and wheat | Penicillium chrysogenum CCT 1273 | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium chrysogenum | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium chrysogenum CCT 1273 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | cf. EC 3.1.3.8 | Penicillium chrysogenum |
| Phy | - |
Penicillium chrysogenum |
| phytase | - |
Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium chrysogenum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range, recombinant enzyme, maximal activity at 50°C, 64% at 40°C | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium chrysogenum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | maximal activity at pH 5.0 followed by pH 2.0 (73% of maximal activity), no activity at pH 7.0-8.0 | Penicillium chrysogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | purified recombinant enzyme, 2 h at room temperature, completely stable | Penicillium chrysogenum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the consensus sequence RHGXRXP and HD, typical of histidine acid phosphatases | Penicillium chrysogenum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
