Application | Comment | Organism |
---|---|---|
food industry | the enzyme can be applied in dephytinizing animal feeds, and the baking industry. Effect of phytase supplementation in different doses on bread characteristics, overview | Enterobacter sp. ACSS |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Enterobacter sp. ACSS | |
cetyltrimethylammoniumbromid | CTAB | Enterobacter sp. ACSS | |
Cu2+ | - |
Enterobacter sp. ACSS | |
DTT | - |
Enterobacter sp. ACSS | |
EDTA | - |
Enterobacter sp. ACSS | |
Fe2+ | - |
Enterobacter sp. ACSS | |
additional information | the enzyme is fairly stable in the presence of pepsin under physiological conditions | Enterobacter sp. ACSS | |
Pb2+ | - |
Enterobacter sp. ACSS | |
SDS | - |
Enterobacter sp. ACSS | |
Triton X-100 | - |
Enterobacter sp. ACSS | |
Tween 20 | - |
Enterobacter sp. ACSS | |
Tween 80 | - |
Enterobacter sp. ACSS | |
Zn2+ | - |
Enterobacter sp. ACSS |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michealis-Menten kinetics | Enterobacter sp. ACSS | |
0.21 | - |
myo-inositol hexakisphosphate | pH 2.5, 60°C | Enterobacter sp. ACSS |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Enterobacter sp. ACSS | |
Mg2+ | activates | Enterobacter sp. ACSS | |
Mn2+ | activates | Enterobacter sp. ACSS |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
- |
Enterobacter sp. ACSS |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Enterobacter sp. ACSS | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. ACSS | - |
isolated from a rhizosphere | - |
Purification (Comment) | Organism |
---|---|
native enzyme 14.22fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity | Enterobacter sp. ACSS |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimization of process parameters for phytase production by Enterobacter sp. ACSS leads to a 4.6fold improvement in submerged fermentation, which is enhanced further in fed-batch fermentation | Enterobacter sp. ACSS | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
805.98 | - |
purified enzyme, pH 2.5, 60°C | Enterobacter sp. ACSS |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hydrolysis of insoluble metal-phytates, overview. The enzyme is also active with 4-nitrophenyl phosphate, sodium diphosphate, glucose-1-phosphate, and glucose-6-phosphate | Enterobacter sp. ACSS | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Enterobacter sp. ACSS | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE | Enterobacter sp. ACSS |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.8 | Enterobacter sp. ACSS |
myo-inositolhexakisphosphate phosphohydrolase | - |
Enterobacter sp. ACSS |
phytase | - |
Enterobacter sp. ACSS |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Enterobacter sp. ACSS |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | activity range | Enterobacter sp. ACSS |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | half-life of the enzyme at 50, 60, 70 and 80°C is 866.2, 693.0, 37.8 and 11.3 min, respectively. The activity is lost completely at 80°C after 55 min incubation | Enterobacter sp. ACSS |
80 | - |
half-life is 11.3 min | Enterobacter sp. ACSS |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1640 | - |
myo-inositol hexakisphosphate | pH 2.5, 60°C | Enterobacter sp. ACSS |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Enterobacter sp. ACSS |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | activity range | Enterobacter sp. ACSS |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, retains complete activity for 120 min | Enterobacter sp. ACSS |
2 | 6 | half-life of the enzyme at pH 2.0, 3.0, 4.0, 5.0 and 6.0 is 577.5, 433.1, 770.0, 247.5 and 91.2 min, respectively | Enterobacter sp. ACSS |
3 | 4 | purified enzyme, loss of 10% activity after 120 min | Enterobacter sp. ACSS |
5 | - |
purified enzyme, loss of 30% activity after 120 min | Enterobacter sp. ACSS |
6 | - |
purified enzyme, loss of 60% activity after 120 min, and of all activity after 240 min | Enterobacter sp. ACSS |
General Information | Comment | Organism |
---|---|---|
physiological function | phytase degrades phytates to release phosphate and other essential ions. It is widespread in nature, found in plants, animals and microorganisms. The use of phytase reduces phosphate excretion in monogastric animals and avoids supplementation of feeds with inorganic phosphate. Animal stomach is the main functional site for phytase-mediated dephosphorylation in the gastrointestinal tract | Enterobacter sp. ACSS |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7809.5 | - |
myo-inositol hexakisphosphate | pH 2.5, 60°C | Enterobacter sp. ACSS |