Literature summary for 3.1.3.26 extracted from

Dionisio, G.; Madsen, C.; Holm, P.; Welinder, K.; Jorgensen, M.; Stoger, E.; Arcalis, E.; Brinch-Pedersen, H.
Cloning and characterization of purple acid phosphatase phytases from wheat, barley, maize, and rice (2011), Plant Physiol., 156, 1087-1100.

Cloned(Commentary)

Cloned (Comment)	Organism
phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium	Oryza sativa Japonica Group

Cloned (Comment)

Organism

phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium

Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	substrate specificity and kinetics of recombinant isozymes, overview	Oryza sativa Japonica Group
0.054	-	myo-inositol-1,2,3,4,5,6-hexakisphosphate	recombinant isozyme b, pH 5.0, 36°C	Oryza sativa Japonica Group

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE	Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	Oryza sativa Japonica Group	-	? + phosphate	-	?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	Oryza sativa Japonica Group Himalaya	-	? + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	D6QSX9	isozyme b; isozyme b	-
Oryza sativa Japonica Group Himalaya	D6QSX9	isozyme b; isozyme b	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites	Oryza sativa Japonica Group

Purification (Commentary)

Purification (Comment)	Organism
soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration	Oryza sativa Japonica Group

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Oryza sativa Japonica Group	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	substrate specificity of the recombinant isozyme, overview	Oryza sativa Japonica Group	?	-	?
additional information	substrate specificity of the recombinant isozyme, overview	Oryza sativa Japonica Group Himalaya	?	-	?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	-	Oryza sativa Japonica Group	? + phosphate	-	?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	-	Oryza sativa Japonica Group Himalaya	? + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE	Oryza sativa Japonica Group

Synonyms

Synonyms	Comment	Organism
PAP	-	Oryza sativa Japonica Group
PAP type I	-	Oryza sativa Japonica Group
PAPhys	-	Oryza sativa Japonica Group
purple acid phosphatase	-	Oryza sativa Japonica Group

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
36	-	assay at	Oryza sativa Japonica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
231	-	myo-inositol-1,2,3,4,5,6-hexakisphosphate	recombinant isozyme b, pH 5.0, 36°C	Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	assay at	Oryza sativa Japonica Group

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
428	-	myo-inositol-1,2,3,4,5,6-hexakisphosphate	recombinant isozyme b, pH 5.0, 36°C	Oryza sativa Japonica Group