Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | 51V-NMR studies show that vanadate is incorporated in the active site of the enzyme. The 6-phytase, which is inactive in the sulfoxidation of thioanisole, show higher affinity for vanadate at pH 7.6 than at pH 5.0. Vanadium is covalently coordinated in the active site of the enzyme to an apical histidine and to oxygen donors. Two sites are available for coordination. Upon addition of H2O2 no peroxide-vanadate-phytase complexes are observed for the 6-phytase as seen for 3-phytase, in agreement with the inactivity of the 6-phytase for the oxidation of thioanisole | Peniophora lycii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peniophora lycii | - |
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Peniophora lycii | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phytase | - |
Peniophora lycii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Peniophora lycii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Peniophora lycii |