Literature summary for 3.1.3.26 extracted from

Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.
Unfolding and refolding of Aspergillus niger PhyB phytase: role of disulfide bridges (2008), J. Agric. Food Chem., 56, 8179-8183.

General Stability

General Stability	Organism
the role of disulfide bridges in the folding of Aspergillus niger PhyB is investigated using dynamic light scattering. Guanidinium chloride (1 M) unfolds phytase. Its removal by dialysis refolds the protein. Tris(2-carboxyethyl)phosphine (TCEP) reduces the refolding activity by 68%. The hydrodynamic radius of PhyB phytase decreases from 5.5 to 4.14 nm when the protein is subjected to 1.0 M GuCl concentration. The active homodimer, is reduced to a monomer	Aspergillus niger

Organism

the role of disulfide bridges in the folding of Aspergillus niger PhyB is investigated using dynamic light scattering. Guanidinium chloride (1 M) unfolds phytase. Its removal by dialysis refolds the protein. Tris(2-carboxyethyl)phosphine (TCEP) reduces the refolding activity by 68%. The hydrodynamic radius of PhyB phytase decreases from 5.5 to 4.14 nm when the protein is subjected to 1.0 M GuCl concentration. The active homodimer, is reduced to a monomer

Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
183000	-	homodimer, dynamic light scattering	Aspergillus niger

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol hexakisphosphate + H2O	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	Aspergillus niger	? + phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	dynamic light scattering, 2*92000	Aspergillus niger

Synonyms

Synonyms	Comment	Organism
PhyB	-	Aspergillus niger
phytase	-	Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
58	-	assay at	Aspergillus niger

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.5	-	-	Aspergillus niger