Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Citrobacter amalonaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | significantly inhibited by 1 mM Ag+ | Citrobacter amalonaticus | |
Fe2+ | significantly inhibited by 1 mM Fe2+ | Citrobacter amalonaticus | |
additional information | highly resistant to pepsin or trypsin treatment | Citrobacter amalonaticus | |
SDS | almost complete inhibition by 1 mM SDS | Citrobacter amalonaticus | |
Zn2+ | significantly inhibited by 1 mM Zn2+ | Citrobacter amalonaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
myo-inositol hexakisphosphate | recombinant enzyme, in 0.1 M sodium acetate buffer (pH 4.5) at 37°C | Citrobacter amalonaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | little enhancement of activity at 1 mM | Citrobacter amalonaticus | |
Co2+ | little enhancement of activity at 1 mM | Citrobacter amalonaticus | |
Mg2+ | little enhancement of activity at 1 mM | Citrobacter amalonaticus | |
Mn2+ | little enhancement of activity at 1 mM | Citrobacter amalonaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46300 | - |
deduced from amino acid sequence | Citrobacter amalonaticus |
49000 | - |
SDS-PAGE, after deglycosylation | Citrobacter amalonaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter amalonaticus | Q003Y3 | - |
- |
Citrobacter amalonaticus CGMCC 1696 | Q003Y3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Citrobacter amalonaticus |
Purification (Comment) | Organism |
---|---|
Sephacryl S-200 HR gel filtration | Citrobacter amalonaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3548 | - |
recombinant enzyme, at 37°C | Citrobacter amalonaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Citrobacter amalonaticus | myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Citrobacter amalonaticus CGMCC 1696 | myo-inositol pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AppA | - |
Citrobacter amalonaticus |
histidine acid phosphatase | - |
Citrobacter amalonaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Citrobacter amalonaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Citrobacter amalonaticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | more than 85% of the total activity is retained after incubation at pH 3.0 to 10.0 for 1 h | Citrobacter amalonaticus |