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Literature summary for 3.1.3.26 extracted from

  • Luo, H.; Huang, H.; Yang, P.; Wang, Y.; Yuan, T.; Wu, N.; Yao, B.; Fan, Y.
    A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris (2007), Curr. Microbiol., 55, 185-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Citrobacter amalonaticus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ significantly inhibited by 1 mM Ag+ Citrobacter amalonaticus
Fe2+ significantly inhibited by 1 mM Fe2+ Citrobacter amalonaticus
additional information highly resistant to pepsin or trypsin treatment Citrobacter amalonaticus
SDS almost complete inhibition by 1 mM SDS Citrobacter amalonaticus
Zn2+ significantly inhibited by 1 mM Zn2+ Citrobacter amalonaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
myo-inositol hexakisphosphate recombinant enzyme, in 0.1 M sodium acetate buffer (pH 4.5) at 37°C Citrobacter amalonaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ little enhancement of activity at 1 mM Citrobacter amalonaticus
Co2+ little enhancement of activity at 1 mM Citrobacter amalonaticus
Mg2+ little enhancement of activity at 1 mM Citrobacter amalonaticus
Mn2+ little enhancement of activity at 1 mM Citrobacter amalonaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46300
-
deduced from amino acid sequence Citrobacter amalonaticus
49000
-
SDS-PAGE, after deglycosylation Citrobacter amalonaticus

Organism

Organism UniProt Comment Textmining
Citrobacter amalonaticus Q003Y3
-
-
Citrobacter amalonaticus CGMCC 1696 Q003Y3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Citrobacter amalonaticus

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-200 HR gel filtration Citrobacter amalonaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3548
-
recombinant enzyme, at 37°C Citrobacter amalonaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Citrobacter amalonaticus myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Citrobacter amalonaticus CGMCC 1696 myo-inositol pentakisphosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
AppA
-
Citrobacter amalonaticus
histidine acid phosphatase
-
Citrobacter amalonaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Citrobacter amalonaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Citrobacter amalonaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 more than 85% of the total activity is retained after incubation at pH 3.0 to 10.0 for 1 h Citrobacter amalonaticus