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Literature summary for 3.1.3.26 extracted from

  • Kim, T.; Mullaney, E.J.; Porres, J.M.; Roneker, K.R.; Crowe, S.; Rice, S.; Ko, T.; Ullah, A.H.; Daly, C.B.; Welch, R.; Lei, X.G.
    Shifting the pH profile of Aspergillus niger PhyA phytase to match the stomach pH enhances its effectiveness as an animal feed additive (2006), Appl. Environ. Microbiol., 72, 4397-4403.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment) Organism
mutants expressed in Pichia pastoris Aspergillus niger

Protein Variants

Protein Variants Comment Organism
D262H increased activity compared to the wild type enzyme Aspergillus niger
E228K shows a shift of the pH optimum to 3.8 and 266% greater hydrolysis of soy phytate at pH 3.5 than the wild type enzyme Aspergillus niger
E228K/K300R/K301E shows large increase in the phytase activity ratio at pH 3.5 over pH 5.5 Aspergillus niger
E228Q decreased activity compared to the wild type enzyme Aspergillus niger
K300E/K301E greater improvement in relative activity at pH 3.5 and lower specific activity than the wild type enzyme Aspergillus niger
K301E increased activity compared to the wild type enzyme Aspergillus niger
K91A decreased activity compared to the wild type enzyme Aspergillus niger
K91E increased activity compared to the wild type enzyme Aspergillus niger
K94E decreased activity compared to the wild type enzyme Aspergillus niger
K94E/K300E/K301E greater improvement in relative activity at pH 3.5 and lower specific activity than the wild type enzyme Aspergillus niger
Q50L increased activity compared to the wild type enzyme Aspergillus niger
Q50P increased activity compared to the wild type enzyme Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0386
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger
0.1773
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger
0.2278
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
0.3219
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + H2O
-
 Aspergillus niger D-fructose + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger myo-inositol pentakisphosphate + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Aspergillus niger p-nitrophenol + phosphate
-
 ?
phosphoenolpyruvate + H2O
-
 Aspergillus niger pyruvate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
6-phytase
-
 Aspergillus niger
PhyA phytase
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52 0.7 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
5.7
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
43.97
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
44.83
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger
80.74
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
123.1
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
 shows a bihump two-pH-optima profile at pH 5.5 and 2.5 Aspergillus niger
5.5
-
 shows a bihump two-pH-optima profile at pH 5.5 and 2.5 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 lowest relative activity at pH 3.5 Aspergillus niger