Protein Variants | Comment | Organism |
---|---|---|
Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E | highly active phytase with no loss of activity after heating at 62°C for 1 h and 27% of its initial activity after 10 min at 85°C, which is a significant improvement over the appA parental phytase. The mutant enzyme also shows a 3.5fold enhancement in gastric stability | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
appA phytase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | - |
1 h, no loss of activity, mutant enzyme Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E | Escherichia coli |
64 | - |
Tm-value for parental phytase is 63.7°C | Escherichia coli |
75.7 | - |
Tm-value for mutant enzyme Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E | Escherichia coli |
85 | - |
10 min, 27% loss of activity,mutant enzyme Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E | Escherichia coli |