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Literature summary for 3.1.3.26 extracted from

  • Cho, J.S.; Lee, C.W.; Kang, S.H.; Lee, J.C.; Bok, J.D.; Moon, Y.S.; Lee, H.G.; Kim, S.C.; Choi, Y.J.
    Purification and characterization of a phytase from Pseudomonas syringae MOK1 (2003), Curr. Microbiol., 47, 290-294.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 5 mM, 70% inhibition Pseudomonas syringae
Cu2+ 5 mM, 70% inhibition Pseudomonas syringae
EDTA 5 mM, 70% inhibition Pseudomonas syringae
Mn2+ 5 mM, 70% inhibition Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
myo-inositol hexakisphosphate pH 5.5, 40°C Pseudomonas syringae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Pseudomonas syringae MOK1
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae ? + phosphate
-
?
myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae MOK1 ? + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Pseudomonas syringae

Synonyms

Synonyms Comment Organism
MOK1 phytase
-
Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas syringae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 30% of maximal activity, 50°C: about 45% of maximal activity Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Pseudomonas syringae

pH Range

pH Minimum pH Maximum Comment Organism
4.2 6.5 pH 4.2: about 80% of maximal activity, pH 6.5: about 70% of maximal activity Pseudomonas syringae