Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Anabaena sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | IMPA1 is moderately sensitive to lithium | Anabaena sp. | |
additional information | not inhibited by Na+ or K+ | Anabaena sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, 1 mM is the optimum concentration | Anabaena sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol phosphate + H2O | Anabaena sp. | highest activity | myo-inositol + phosphate | - |
? | |
myo-inositol phosphate + H2O | Anabaena sp. PCC 7120 | highest activity | myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | - |
- |
- |
Anabaena sp. PCC 7120 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Anabaena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows 5-20% activity for D-myo-inositol 1,4-bisphophate, D-fructose 1,6-diphosphate, 3'-phosphoadenosine 5'-phosphate, 3'-phospho phosphoadenosine-5?-phosphosulfate, ATP, AMP, ADP and NADP+ compared to myo-inositol phosphate | Anabaena sp. | ? | - |
? | |
additional information | the enzyme shows 5-20% activity for D-myo-inositol 1,4-bisphophate, D-fructose 1,6-diphosphate, 3'-phosphoadenosine 5'-phosphate, 3'-phospho phosphoadenosine-5?-phosphosulfate, ATP, AMP, ADP and NADP+ compared to myo-inositol phosphate | Anabaena sp. PCC 7120 | ? | - |
? | |
myo-inositol phosphate + H2O | highest activity | Anabaena sp. | myo-inositol + phosphate | - |
? | |
myo-inositol phosphate + H2O | highest activity | Anabaena sp. PCC 7120 | myo-inositol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
All2917 | - |
Anabaena sp. |
IMPA1 | - |
Anabaena sp. |
IMPase | - |
Anabaena sp. |
inositol monophosphatase | - |
Anabaena sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Anabaena sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Anabaena sp. |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5 | - |
at pH 8.0 and 30°C | Anabaena sp. | Li+ |
Organism | Comment | Expression |
---|---|---|
Anabaena sp. | the transcription of the enzyme is significantly upregulated upon sucrose-mediated osmotic stress (treatment with 500 mM sucrose for up to 4 h) | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in osmotic adaptation against stress caused by sucrose or sorbitol | Anabaena sp. |