Literature summary for 3.1.3.25 extracted from

Arai, R.; Ito, K.; Ohnishi, T.; Ohba, H.; Akasaka, R.; Bessho, Y.; Hanawa-Suetsugu, K.; Yoshikawa, T.; Shirouzu, M.; Yokoyama, S.
Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures (2007), Proteins, 67, 732-742.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, at 20°C, with 10 mM potassium dihydrogen phosphate, 15% PEG 8000, 20% glycerol, and 2 mM MgSO4 for crystal 1, 10 mM potassium dihydrogen phosphate, 12% PEG 8000, 20% glycerol, and 2 mM MgSO4 for crystal 2, 25 mM potassium dihydrogen phosphate, 14% PEG 8000, and 5 mM LiCl for crystal 3, and 8 mM potassium dihydrogen phosphate, 14% PEG 8000, 20% glycerol, and 5 mM CaCl2 for crystal 4	Homo sapiens

Crystallization (Comment)

Organism

sitting drop vapour diffusion method, at 20°C, with 10 mM potassium dihydrogen phosphate, 15% PEG 8000, 20% glycerol, and 2 mM MgSO4 for crystal 1, 10 mM potassium dihydrogen phosphate, 12% PEG 8000, 20% glycerol, and 2 mM MgSO4 for crystal 2, 25 mM potassium dihydrogen phosphate, 14% PEG 8000, and 5 mM LiCl for crystal 3, and 8 mM potassium dihydrogen phosphate, 14% PEG 8000, 20% glycerol, and 5 mM CaCl2 for crystal 4

Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycerol 2-phosphate + H2O	-	Homo sapiens	glycerol + phosphate	-	?
L-myo-inositol 1-phosphate + H2O	-	Homo sapiens	L-myo-inositol + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	in solution, analytical ultracentrifugation	Homo sapiens

Synonyms

Synonyms	Comment	Organism
IMPA2	-	Homo sapiens
myo-inositol monophosphatase	-	Homo sapiens
myo-inositol monophosphatase 2	-	Homo sapiens