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Literature summary for 3.1.3.25 extracted from
- Inositol-1 (or 4)-monophosphatase from Glycine max embryo axes is a phosphatase with broad substrate specificity that includes phytate dephosphorylation (2007), Biochim. Biophys. Acta, 1770, 543-550.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | - |
Glycine max |  |
| additional information | not inhibited by butanedione monoxime, or orthovanadate | Glycine max | |
| NaF | - |
Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.235 | - |
diphosphate | at 37°C, in 25 mM phosphate buffer with 2 mM dithiothreitol (pH 7.0) and 2 mM MgCl2 | Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Glycine max | |
| Co2+ | activates | Glycine max | |
| Cu2+ | activates | Glycine max | |
| Mg2+ | activates | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
SDS-PAGE | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S-300 gel filtration, hydroxyapatite column chromatography, and DEAE-Sephacel column chromatography | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Glycine max | - |
| hypocotyl | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.37 | - |
total cell extract, at 37°C, using sodium diphosphate supplemented with MgCl2 as a substrate | Glycine max |
| 375.6 | - |
after 85.94fold purification, at 37°C, using sodium diphosphate supplemented with MgCl2 as a substrate | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Glycine max | ADP + phosphate | - |
? | |
| D-myo-inositol 1,2,3,4,5,6-hexakisphosphate + H2O | - |
Glycine max | D-myo-inositol 1,2,3,4,5-pentakisphosphate + phosphate | - |
? | |
| D-myo-inositol 1-phosphate + H2O | - |
Glycine max | D-myo-inositol + phosphate | - |
? | |
| diphosphate + H2O | - |
Glycine max | 2 phosphate | - |
? | |
| additional information | does not hydrolyze p-nitrophenyl phosphate, ADP, AMP or D-glucose 6-phosphate | Glycine max | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMPase | - |
Glycine max |
| inositol-1(or 4)-monophosphatase | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 80 | - |
Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Glycine max |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Glycine max | isoelectric focusing | - |
5.9 |
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Release 2023.1 (January 2023)
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