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Literature summary for 3.1.3.25 extracted from

  • Nigou, J.; Besra, G.S.
    Characterization and regulation of inositol monophosphatase activity in Mycobacterium smegmatis (2002), Biochem. J., 361, 385-390.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.25

Inhibitors

Inhibitors Comment Organism Structure
Li+ 50% inhibition at 0.8 mM, also able to inhibit cell growth Mycolicibacterium smegmatis
myo-inositol product inhibition, competitive Mycolicibacterium smegmatis
Na+ 50% inhibition at 70 mM Mycolicibacterium smegmatis
phosphate product inhibition, competitive Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
 Mg2+ pH 7.5, 37°C Mycolicibacterium smegmatis
66
-
 myo-inositol 1-phosphate pH 7.5, 37°C Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, highest activity at 6 mM Mg2+, inhibitory at higher concentrations Mycolicibacterium smegmatis
Zn2+ no effect between 0.01-10 mM Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol 1-phosphate + H2O Mycolicibacterium smegmatis key metabolic step, de novo synthesis of myo-inositol, phosphatidylinositol is essential of growth myo-inositol + phosphate
-
 ?
myo-inositol 1-phosphate + H2O Mycolicibacterium smegmatis mc(2)155 key metabolic step, de novo synthesis of myo-inositol, phosphatidylinositol is essential of growth myo-inositol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis mc(2)155
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 when grown on glycerol highest activity at the end of exponential growth phase, when grown on glucose highest activity in the middle of exponential growth phase Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol 1-phosphate + H2O
-
 Mycolicibacterium smegmatis myo-inositol + phosphate
-
 ?
myo-inositol 1-phosphate + H2O key metabolic step, de novo synthesis of myo-inositol, phosphatidylinositol is essential of growth Mycolicibacterium smegmatis myo-inositol + phosphate
-
 ?
myo-inositol 1-phosphate + H2O
-
 Mycolicibacterium smegmatis mc(2)155 myo-inositol + phosphate
-
 ?
myo-inositol 1-phosphate + H2O key metabolic step, de novo synthesis of myo-inositol, phosphatidylinositol is essential of growth Mycolicibacterium smegmatis mc(2)155 myo-inositol + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Mycolicibacterium smegmatis

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Mycolicibacterium smegmatis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7
-
 phosphate pH 7.5, 37°C Mycolicibacterium smegmatis
39
-
 myo-inositol pH 7.5, 37°C Mycolicibacterium smegmatis