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Literature summary for 3.1.3.23 extracted from

  • Ye, J.J.; Saier, M.H.
    Purification and characterization of a small membrane-associated sugar phosphate phosphatase that is allosterically activated by HPr(Ser(P)) of the phosphotransferase system in Lactococcus lactis (1995), J. Biol. Chem., 270, 16740-16744.
    View publication on PubMed
Search for more literature for 3.1.3.23

Activating Compound

Activating Compound Comment Organism Structure
S46D HPr i.e. mutant phosphocarrier protein, 10fold activation of sugar phosphate phosphatase I Lactococcus lactis
S46D HPr Km: 0.007 mM Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
50
-
 mannitol 1-phosphate
-
 Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Lactococcus lactis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis
Fe2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis
Mg2+ the enzyme is dependent on divalent cations, Mg2+ is most effective, half-maximal stimulation at 1 mM Lactococcus lactis
Mn2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis
Zn2+ stimulates to a lesser degree than Mg2+ Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 sugar phosphate phosphatase II
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-diphosphoglycerate + H2O
-
 Lactococcus lactis ?
-
 ?
2-Deoxy-D-glucose 6-phosphate + H2O
-
 Lactococcus lactis 2-Deoxy-D-glucose + phosphate
-
 ?
alpha-D-glucose 1-phosphate + H2O
-
 Lactococcus lactis alpha-D-glucose + phosphate
-
 ?
beta-D-methyl-thiogalactopyranoside 6-phosphate + H2O
-
 Lactococcus lactis beta-D-methyl-thiogalactopyranoside + phosphate
-
 ?
D-fructose 1-phosphate + H2O
-
 Lactococcus lactis D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O
-
 Lactococcus lactis D-fructose + phosphate
-
 ?
D-galactose 6-phosphate + H2O
-
 Lactococcus lactis D-galactose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Lactococcus lactis D-glucose + phosphate
-
 ?
D-mannose 6-phosphate + H2O
-
 Lactococcus lactis D-mannose + phosphate
-
 ?
mannitol 1-phosphate + H2O
-
 Lactococcus lactis mannitol + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 10 min, unaffected Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 hydrolysis of mannitol 1-phosphate, with or without the activator S46D HPr Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 about 60% of maximal activity at pH 5.0 and at pH 9.0, hydrolysis of mannitol 1-phosphate Lactococcus lactis