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Literature summary for 3.1.3.23 extracted from

  • Lee, Y.P.; Lee, A.T.; Choy, F.Y.M.; Sowokinos, J.R.
    Molecular weight and some physicochemical and catalytic properties of sugar phosphate phosphohydrolase from Escherichia coli and Neisseria meningitidis (1975), J. Biol. Chem., 250, 3729-3737.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Dodecyl sulfate
-
Escherichia coli
Dodecyl sulfate
-
Neisseria meningitidis
glucose 1-phosphate complete inhibition of hydrolysis of glucose 6-phosphate as long as glucose 1-phosphate to glucose 6-phosphate ratios are greater than 0.12 Escherichia coli
glucose 1-phosphate complete inhibition of hydrolysis of glucose 6-phosphate as long as glucose 1-phosphate to glucose 6-phosphate ratios are greater than 0.12 Neisseria meningitidis
guanidine
-
Escherichia coli
guanidine
-
Neisseria meningitidis
mannose 1-phosphate strong competitive inhibitor of hydrolysis of glucose 6-phosphate Escherichia coli
p-mercuribenzoate
-
Escherichia coli
p-mercuribenzoate
-
Neisseria meningitidis
Urea
-
Escherichia coli
Urea
-
Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
D-glucuronate 1-phosphate
-
Escherichia coli
2.1
-
alpha-D-xylose 1-phosphate
-
Escherichia coli
2.2
-
alpha-D-gentiobiose 1-phosphate
-
Escherichia coli
2.2
-
alpha-D-maltose 1-phosphate
-
Escherichia coli
2.3
-
alpha-D-lactose 1-phosphate
-
Escherichia coli
2.5
-
alpha-D-glucose 1-phosphate
-
Escherichia coli
2.5
-
alpha-D-galactose 1-phosphate
-
Escherichia coli
2.5
-
alpha-D-mannose 1-phosphate
-
Escherichia coli
2.8
-
D-Fructose 1-phosphate
-
Escherichia coli
3
-
D-fructose 6-phosphate
-
Escherichia coli
3
-
D-glucosamine 6-phosphate
-
Escherichia coli
3
-
alpha-D-mannose 1-phosphate
-
Escherichia coli
3.1
-
D-glucose 6-phosphate
-
Escherichia coli
3.1
-
2-deoxy-D-glucose 6-phosphate
-
Escherichia coli
3.1
-
D-galactose 6-phosphate
-
Escherichia coli
3.3
-
D-ribose 5-phosphate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
2 * 51000, SDS-PAGE Escherichia coli
100000
-
gel filtration Escherichia coli
100000
-
gel filtration Neisseria meningitidis
100000
-
low speed sedimentation Neisseria meningitidis
101000
-
low speed sedimentation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Neisseria meningitidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.81
-
-
Neisseria meningitidis

Storage Stability

Storage Stability Organism
1-3°C, pH 10, stable for 12 weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Deoxy-D-glucose 6-phosphate + H2O
-
Escherichia coli 2-Deoxy-D-glucose + phosphate
-
?
2-Deoxy-D-glucose 6-phosphate + H2O
-
Neisseria meningitidis 2-Deoxy-D-glucose + phosphate
-
?
alpha-cellobiose 1-phosphate + H2O
-
Escherichia coli alpha-cellobiose + phosphate
-
?
alpha-D-galactose 1-phosphate + H2O
-
Escherichia coli alpha-D-galactose + phosphate
-
?
alpha-D-galactose 1-phosphate + H2O
-
Neisseria meningitidis alpha-D-galactose + phosphate
-
?
alpha-D-gentiobiose 1-phosphate + H2O
-
Escherichia coli alpha-D-gentiobiose + phosphate
-
?
alpha-D-glucosamine 1-phosphate + H2O
-
Escherichia coli alpha-D-glucosamine + phosphate
-
?
alpha-D-glucose 1-phosphate + H2O
-
Escherichia coli alpha-D-glucose + phosphate
-
?
alpha-D-glucose 1-phosphate + H2O
-
Neisseria meningitidis alpha-D-glucose + phosphate
-
?
alpha-D-glucuronic acid 1-phosphate + H2O
-
Escherichia coli alpha-D-glucuronic acid + phosphate
-
?
alpha-D-glucuronic acid 1-phosphate + H2O
-
Neisseria meningitidis alpha-D-glucuronic acid + phosphate
-
?
alpha-D-lactose 1-phosphate + H2O
-
Escherichia coli alpha-D-lactose + phosphate
-
?
alpha-D-mannose 1-phosphate + H2O
-
Escherichia coli alpha-D-mannose + phosphate
-
?
alpha-D-mannose 1-phosphate + H2O
-
Neisseria meningitidis alpha-D-mannose + phosphate
-
?
alpha-D-xylose 1-phosphate + H2O
-
Escherichia coli alpha-D-xylose + phosphate
-
?
alpha-gentiobiose 1-phosphate + H2O
-
Escherichia coli alpha-gentiobiose + phosphate
-
?
alpha-lactose 1-phosphate + H2O
-
Escherichia coli alpha-lactose + phosphate
-
?
alpha-lactose 1-phosphate + H2O
-
Neisseria meningitidis alpha-lactose + phosphate
-
?
alpha-maltose 1-phosphate + H2O
-
Escherichia coli alpha-maltose + phosphate
-
?
D-fructose 1,6-diphosphate + H2O
-
Escherichia coli ?
-
?
D-fructose 1,6-diphosphate + H2O
-
Neisseria meningitidis ?
-
?
D-fructose 1-phosphate + H2O
-
Escherichia coli D-fructose + phosphate
-
?
D-fructose 1-phosphate + H2O
-
Neisseria meningitidis D-fructose + phosphate
-
?
D-fructose 6-phosphate + H2O
-
Escherichia coli D-fructose + phosphate
-
?
D-fructose 6-phosphate + H2O
-
Neisseria meningitidis D-fructose + phosphate
-
?
D-galactose 6-phosphate + H2O
-
Escherichia coli D-galactose + phosphate
-
?
D-galactose 6-phosphate + H2O
-
Neisseria meningitidis D-galactose + phosphate
-
?
D-glucosamine 6-phosphate + H2O
-
Escherichia coli D-glucosamine + phosphate
-
?
D-glucose 6-phosphate + H2O
-
Escherichia coli D-glucose + phosphate
-
?
D-glucose 6-phosphate + H2O
-
Neisseria meningitidis D-glucose + phosphate
-
?
D-glucuronate 1-phosphate + H2O
-
Escherichia coli D-glucuronate + phosphate
-
?
D-mannose 6-phosphate + H2O
-
Escherichia coli D-mannose + phosphate
-
?
D-mannose 6-phosphate + H2O
-
Neisseria meningitidis D-mannose + phosphate
-
?
D-ribose 5-phosphate + H2O
-
Escherichia coli D-ribose + phosphate
-
?
D-ribose 5-phosphate + H2O
-
Neisseria meningitidis D-ribose + phosphate
-
?
D-xylose 1-phosphate + H2O
-
Escherichia coli D-xylose + phosphate
-
?
D-xylose 1-phosphate + H2O
-
Neisseria meningitidis D-xylose + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 51000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 3.0-9.7, stable for at least 40 min, not stable above pH 10 or below pH 2.5 Escherichia coli
55
-
pH 6, 2 min, stable up to Escherichia coli
60
-
pH 4.0-9.0, 2 min, quite stable. At pH 6.0, 20% loss of activity after 2 min. Complete loss of activity at pH 1.0, 2.0, 3.0 or 10.0 after 2 min Escherichia coli
65
-
pH 6.0, 2 min, 80% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
hydrolysis of glucose 1-phosphate or glucose 6-phosphate Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 55% of maximal activity, pH 8.0: about 45% of maximal activity, hydrolysis of glucose 1 -phosphate Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9.7 30°C, stable for at least 40 min Escherichia coli
4 9 60°C, 2 min, quite stable Escherichia coli
6
-
stable up to 55°C. At 60°C, 2 min, about 20% loss of activity. At 65°C, 2 min, 80% loss of activity Escherichia coli