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Literature summary for 3.1.3.23 extracted from
- Identification of a new phosphatase enzyme potentially involved in the sugar phosphate stress response in Pseudomonas fluorescens (2017), Appl. Environ. Microbiol., 83, e02361 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | addition of MN2+ decreaseds the reaction rate both without and in combination with Mg2x05 | Pseudomonas fluorescens |  |
| NaCl | 50 mM, inhibitory | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
D-fructose 6-phosphate | pH 7.5, 23°C | Pseudomonas fluorescens | |
| 1.8 | - |
D-glucose 6-phosphate | pH 7.5, 23°C | Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimum concentration 2.5 mM | Pseudomonas fluorescens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | C3K9U8 | - |
- |
| Pseudomonas fluorescens SBW25 | C3K9U8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Pseudomonas fluorescens SBW25 | 4-nitrophenol + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Pseudomonas fluorescens | D-fructose + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Pseudomonas fluorescens SBW25 | D-fructose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Pseudomonas fluorescens | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Pseudomonas fluorescens SBW25 | D-glucose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | preferred substrate | Pseudomonas fluorescens | D-ribose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | preferred substrate | Pseudomonas fluorescens SBW25 | D-ribose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29900, calculated from sequence and SDS-PAGE, recombinant protein including His-tag | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFLU2693 | - |
Pseudomonas fluorescens |
| SPP | - |
Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.6 | - |
D-fructose 6-phosphate | pH 7.5, 23°C | Pseudomonas fluorescens | |
| 11.4 | - |
D-glucose 6-phosphate | pH 7.5, 23°C | Pseudomonas fluorescens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | deletion of the Spp gene does not affect growth of the wild-type strain on fructose, but the gene cannot be deleted in the alginate-producing strain. Sugar phosphate accumulation in Spp mutant strains causes diminished growth in some of the strains, which is partially relieved by Spp overexpression. Overexpression of Spp in fructose-grown alginate-producing strains negatively affects both growth and alginate production | Pseudomonas fluorescens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.4 | - |
D-glucose 6-phosphate | pH 7.5, 23°C | Pseudomonas fluorescens | |
| 13 | - |
D-fructose 6-phosphate | pH 7.5, 23°C | Pseudomonas fluorescens | |
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Release 2023.1 (January 2023)
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