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Literature summary for 3.1.3.22 extracted from

  • Liberator, P.; Anderson, J.; Feiglin, M.; Sardana, M.; Griffin, P.; Schmatz, D.; Myers, R.W.
    Molecular cloning and functional expression of mannitol-1-phosphatase from the apicomplexan parasite Eimeria tenella (1998), J. Biol. Chem., 273, 4237-4244.
    View publication on PubMed
Search for more literature for 3.1.3.22

Application

Application Comment Organism
medicine lack of mannitol metabolism in higher eukaryotes Eimeria tenella

Cloned(Commentary)

Cloned (Comment) Organism
cDNA from total RNA from unsporulated oocytes Eimeria tenella

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate at pH 6-8, due to histidine modification Eimeria tenella
NaCl inhibits native M1Pase with an IC50 value of 0.028 mM, for recombinant enzymes between 0.054 mM and 0.044 mM Eimeria tenella

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Eimeria tenella 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34650
-
 LC-ESI-MS analysis Eimeria tenella
34740
-
 calculation from sequence of cDNA Eimeria tenella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol 1-phosphate + H2O Eimeria tenella mannitol metabolism D-mannitol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Eimeria tenella
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification by cDNA in comparison to the biochemical purified enzyme, active side H82 phosphorylated, detected by diethyl pyrocarbonate inhibition and reduced inhibition by p-nitrophenylphosphate Eimeria tenella

Purification (Commentary)

Purification (Comment) Organism
biochemical purification of native enzyme Eimeria tenella
recombinant fusion and His-tagged proteins Eimeria tenella

Source Tissue

Source Tissue Comment Organism Textmining
oocyte unsporulated Eimeria tenella
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol 1-phosphate + H2O mannitol metabolism Eimeria tenella D-mannitol + phosphate
-
 ?
mannitol 1-phosphate + H2O
-
 Eimeria tenella mannitol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer SDS-PAGE and western blot analysis Eimeria tenella

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.028
-
 inhibits native M1Pase with an IC50 value of 0.028 mM, for recombinant enzymes between 0.054 mM and 0.044 mM Eimeria tenella NaCl