BRENDA - Enzyme Database show
show all sequences of 3.1.3.21

Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-acti

Norbeck, J.; Pahlman, A.; Akhtar, N.; Blomberg, A.; Adler, L.; J. Biol. Chem. 271, 13875-1388 (1996)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1
-
DL-glycerol-3-phosphate
for Gpp1p
Saccharomyces cerevisiae
3.9
-
DL-glycerol-3-phosphate
for Gpp2p
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
strict specificity avoids interference with plethora of phosphorylated metabolites in the metabolic network
Saccharomyces cerevisiae
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27800
-
Gpp2p, calculation from sequence of cDNA
Saccharomyces cerevisiae
30400
-
Gpp1p, calculation from sequence of cDNA
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol-3-phosphate + H2O
Saccharomyces cerevisiae
glycerol metabolism
glycerol + phosphate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
baker`s yeast
-
Purification (Commentary)
Commentary
Organism
gel filtration and ion exchange chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
46
-
enzyme Gpp2p
Saccharomyces cerevisiae
49
-
enzyme Gpp1p
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DL-glycerol-3-phosphate + H2O
no stereospecifity
81025
Saccharomyces cerevisiae
glycerol + phosphate
-
81025
Saccharomyces cerevisiae
?
glycerol-3-phosphate + H2O
glycerol metabolism
81025
Saccharomyces cerevisiae
glycerol + phosphate
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
gel filtration and non-denaturating gradient gel electrophoresis
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
enzymes Gpp1p and Gpp2p
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1
-
DL-glycerol-3-phosphate
for Gpp1p
Saccharomyces cerevisiae
3.9
-
DL-glycerol-3-phosphate
for Gpp2p
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
strict specificity avoids interference with plethora of phosphorylated metabolites in the metabolic network
Saccharomyces cerevisiae
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27800
-
Gpp2p, calculation from sequence of cDNA
Saccharomyces cerevisiae
30400
-
Gpp1p, calculation from sequence of cDNA
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol-3-phosphate + H2O
Saccharomyces cerevisiae
glycerol metabolism
glycerol + phosphate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration and ion exchange chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
46
-
enzyme Gpp2p
Saccharomyces cerevisiae
49
-
enzyme Gpp1p
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DL-glycerol-3-phosphate + H2O
no stereospecifity
81025
Saccharomyces cerevisiae
glycerol + phosphate
-
81025
Saccharomyces cerevisiae
?
glycerol-3-phosphate + H2O
glycerol metabolism
81025
Saccharomyces cerevisiae
glycerol + phosphate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
gel filtration and non-denaturating gradient gel electrophoresis
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
enzymes Gpp1p and Gpp2p
Saccharomyces cerevisiae
Other publictions for EC 3.1.3.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749467
Xu
Discovery and functional char ...
Saccharomyces cerevisiae
ACS Chem. Biol.
13
3011-3020
2018
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729248
Larrouy-Maumus
Chemical mechanism of glycerol ...
Mycobacterium tuberculosis
Biochemistry
53
143-151
2014
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729762
Ditlecadet
Glycerol synthesis in freeze-r ...
Osmerus mordax
Fish Physiol. Biochem.
40
257-266
2014
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2
1
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6
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730667
Caparros-Martin
The kinetic analysis of the su ...
Arabidopsis thaliana
Planta
240
479-487
2014
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730664
Caparros-Martin
HAD hydrolase function unveile ...
Arabidopsis thaliana
Planta
237
943-954
2013
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730818
Larrouy-Maumus
Discovery of a glycerol 3-phos ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
110
11320-11325
2013
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11
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5
730083
Lindner
Glycerol-3-phosphatase of Cory ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
J. Biotechnol.
159
216-224
2012
1
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1
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6
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6
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30
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2
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1
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685707
Popp
Fermentative production of L-g ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
100
497-505
2008
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687860
Zheng
Production of glycerol from gl ...
Saccharomyces cerevisiae
J. Biosci. Bioeng.
105
508-512
2008
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681523
Nguyen
Formation of glycerol from glu ...
Rattus norvegicus
J. Neurochem.
101
1694-1700
2007
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681758
Meynial Salles
Evolution of a Saccharomyces c ...
Escherichia coli
Metab. Eng.
9
152-159
2007
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682390
Caparros-Martin
Arabidopsis thaliana AtGppl an ...
Arabidopsis thaliana
Plant Mol. Biol.
63
505-517
2007
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669659
Driedzic
Glycerol production in rainbow ...
Osmerus mordax
J. Exp. Biol.
209
1016-1023
2006
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664991
Fan
Disruption of a gene encoding ...
Candida albicans
FEMS Microbiol. Lett.
245
107-116
2005
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652171
Pahlman
The yeast glycerol 3-phosphata ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
3555-3563
2001
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81025
Norbeck
Purification and characterizat ...
Saccharomyces cerevisiae
J. Biol. Chem.
271
13875-1388
1996
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81031
Belmans D.; Van Laere
-
Glycerol-3-phosphatase and the ...
Dunaliella tertiolecta
Arch. Microbiol.
150
109-112
1988
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81033
Van Schaftingen
Glycerol formation after the b ...
Phycomyces blakesleeanus
Eur. J. Biochem.
148
399-404
1985
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95139
Paltauf
Utilization of exogenous glyce ...
Saccharomyces uvarum
Biochim. Biophys. Acta
835
322-330
1985
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81026
Gimmler
-
The chloride sensitivity of Du ...
Dunaliella parva
Z. Pflanzenphysiol.
114
131-150
1983
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81027
Brown
-
Osmoregulation in Dunaliellea. ...
Dunaliella tertiolecta
Z. Naturforsch. C
37
1115-1123
1982
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81028
Gimmler
-
The intracellular distribution ...
Dunaliella parva
Z. Naturforsch. C
37
1107-1114
1982
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1
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2
1
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1
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1
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2
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1
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-
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81029
Ben-Amotz
-
Glycerol production by Dunalie ...
Asteromonas gracilis, Dunaliella salina
Experientia
38
49-52
1982
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2
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2
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2
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2
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4
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2
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2
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2
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4
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81030
Sussmann
-
Characterization and partial p ...
Dunaliella salina
Biochim. Biophys. Acta
661
199-204
1981
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1
10
1
1
2
1
1
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1
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1
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1
1
2
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1
1
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1
-
10
-
1
1
2
1
1
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1
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1
1
2
-
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1
1
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81034
Gancedo
Glycerol metabolism in yeasts. ...
Cyberlindnera jadinii
Eur. J. Biochem.
5
165-172
1968
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1
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1
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2
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1
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2
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81035
Schmidt
-
Nonspecific acid phosphomonoes ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
5
37-48
1961
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-
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4
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1
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1
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1
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2
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1
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4
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1
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1
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2
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1
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-
81036
Tsuboi
Acid phosphatase ...
Saccharomyces cerevisiae
J. Biol. Chem.
224
621-635
1956
2
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1
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1
1
1
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1
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2
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1
1
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2
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1
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1
1
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1
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2
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1
1
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