Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TB1 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
DL-glycerol 3-phosphate | Gpp2, in 20 mM Tris-HCl pH 7.0, 5 mM MgCl2, and 10 mM DL-glycerol-3-phosphate | Arabidopsis thaliana | |
5.2 | - |
DL-glycerol 3-phosphate | Gpp1, in 20 mM Tris-HCl pH 7.0, 5 mM MgCl2, and 10 mM DL-glycerol-3-phosphate | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
cytoplasm | Gpp2 | Arabidopsis thaliana | 5737 | - |
plastid | Gpp1 | Arabidopsis thaliana | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
SDS-PAGE, Gpp2 | Arabidopsis thaliana |
33000 | - |
SDS-PAGE, Gpp1 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
meristem | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glycerol 3-phosphate + H2O | - |
Arabidopsis thaliana | glycerol + phosphate | - |
? | |
additional information | poor activity is observed on glucose-6-phosphate (less than 3%), fructose-6-phosphate (less than 12%), or 2-deoxyglucose-6-phosphate (less than 2%) compared to DL-glycerol-3-phosphate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gpp2 | - |
Arabidopsis thaliana |
Gppl | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | isoelectric focusing, Gpp2 | - |
5.6 |
Arabidopsis thaliana | isoelectric focusing, Gpp1 | - |
7.8 |