Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 137.6% activity at 5 mM | Trichoderma harzianum | |
sodium tartrate | 114.4% activity at 5 mM | Trichoderma harzianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 93.5% residual activity at 5 mM | Trichoderma harzianum | |
phosphate | 33% residual activity at 5 mM | Trichoderma harzianum | |
tungstate | 1% residual activity at 5 mM | Trichoderma harzianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000027 | - |
4-nitrophenyl phosphate | at pH 3.8 and 65°C | Trichoderma harzianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require metal divalent ions | Trichoderma harzianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
326000 | - |
dynamic light scattering | Trichoderma harzianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
- |
- |
Trichoderma harzianum ALL42 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Trichoderma harzianum |
Purification (Comment) | Organism |
---|---|
HiTrap phenyl column chromatography and Superdex 200 gel filtration | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | 100% activity | Trichoderma harzianum | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | 100% activity | Trichoderma harzianum ALL42 | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | 39% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | AMP + phosphate | - |
? | |
AMP + H2O | 30% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | adenosine + phosphate | - |
? | |
AMP + H2O | 30% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum ALL42 | adenosine + phosphate | - |
? | |
ATP + H2O | 25.3% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | ADP + phosphate | - |
? | |
ATP + H2O | 25.3% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum ALL42 | ADP + phosphate | - |
? | |
beta-D-glucose 1-phosphate + H2O | 35.5% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | beta-D-glucose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | 33% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | D-fructose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | 33% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum ALL42 | D-fructose + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | 49.4% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | D-glucose + phosphate | - |
? | |
phenyl phosphate + H2O | 52.3% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | phenol + phosphate | - |
? | |
phenyl phosphate + H2O | 52.3% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum ALL42 | phenol + phosphate | - |
? | |
phytic acid + H2O | 20.5% activity compared to 4-nitrophenyl phosphate | Trichoderma harzianum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 90000, SDS-PAGE | Trichoderma harzianum |
Synonyms | Comment | Organism |
---|---|---|
ACPase II | - |
Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Trichoderma harzianum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | about 30% activity at 35°C, about 40% activity at 40°C, about 60% activity at 45-50°C, about 65% activity at 55°C, about 92% activity at 60°C, 100% activity at 65°C, about 96% activity at 70°C, less than 10% activity at 80°C | Trichoderma harzianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the enzyme is stable at 55°C for 120 min, retaining 60% of its activity. The enzyme shows 61%, 48% and 27% activity after 60 min at 50°C, 55°C, and 60°C, respectively | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | - |
- |
Trichoderma harzianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.2 | 5.2 | more than 70% activity between pH 3.2 and 5.2 | Trichoderma harzianum |