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Literature summary for 3.1.3.2 extracted from
- Purification and biochemical characterisation of acid phosphatase-I from seeds of Nelumbo nucifera (2016), Nat. Prod. Res., 30, 570-573 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cr6+ | weak inhibitor | Nelumbo nucifera |  |
| Cu2+ | weak inhibitor | Nelumbo nucifera | |
| Mo2+ | weak inhibitor | Nelumbo nucifera | |
| W2+ | strong inhibitor | Nelumbo nucifera | |
| Zn2+ | strong inhibitor | Nelumbo nucifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.132 | - |
4-nitrophenyl phosphate | at pH 5.0 and 50°C | Nelumbo nucifera | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ exhibits moderate stimulatory activity | Nelumbo nucifera | |
| additional information | no change in enzyme activity was observed in the presence of Na+, Ca2+, and Li+ | Nelumbo nucifera |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Nelumbo nucifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nelumbo nucifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange column chromatography and gel filtration | Nelumbo nucifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Nelumbo nucifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Nelumbo nucifera | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE | Nelumbo nucifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid phosphatase-I | - |
Nelumbo nucifera |
| Apase-I | - |
Nelumbo nucifera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Nelumbo nucifera |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | 80 | after 1 h incubation, the enzyme retains 95% activity at 26°C, 71% activity at 40°C, 50% activity at 50°C, 10% activity at 60°C and loses all of its activity at 80°C | Nelumbo nucifera |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.7 | - |
4-nitrophenyl phosphate | at pH 5.0 and 50°C | Nelumbo nucifera | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Nelumbo nucifera |
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