Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Macrotyloma uniflorum | |
Hg2+ | - |
Macrotyloma uniflorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.934 | - |
4-nitrophenyl phosphate | at pH 5.0 and 50°C | Macrotyloma uniflorum | |
5 | - |
ATP | at pH 5.0 and 50°C | Macrotyloma uniflorum | |
8.7 | - |
ADP | at pH 5.0 and 50°C | Macrotyloma uniflorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Macrotyloma uniflorum | |
Mg2+ | enzyme activity is slightly increased in the presence of Mg2+ ions | Macrotyloma uniflorum | |
additional information | K+, Ca2+ and Na+ have no effect on the enzyme activity | Macrotyloma uniflorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Macrotyloma uniflorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrotyloma uniflorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
UNOsphere-S column chromatography and Sephadex G-100 gel filtration | Macrotyloma uniflorum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Macrotyloma uniflorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Macrotyloma uniflorum | 1-naphthol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | high activity | Macrotyloma uniflorum | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | high activity | Macrotyloma uniflorum | AMP + phosphate | - |
? | |
ATP + H2O | high activity | Macrotyloma uniflorum | ADP + phosphate | - |
? | |
beta-glycerophosphate + H2O | - |
Macrotyloma uniflorum | glycerol + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | low activity | Macrotyloma uniflorum | D-fructose + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | low activity | Macrotyloma uniflorum | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | low activity | Macrotyloma uniflorum | D-glucose + phosphate | - |
? | |
phenyl phosphate + H2O | high activity | Macrotyloma uniflorum | phenol + phosphate | - |
? | |
pyridoxyl 5'-phosphate + H2O | low activity | Macrotyloma uniflorum | pyridoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 28241 + 1 * 27093, MALDI-TOF mass spectrometry | Macrotyloma uniflorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Macrotyloma uniflorum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | more than 50% activity between 20 and 70°C | Macrotyloma uniflorum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 80 | the enzyme is stable up to 55°C. Further heating of the enzyme results in about 45% loss in enzyme activity at 60°C with a total loss in activity at 80°C | Macrotyloma uniflorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Macrotyloma uniflorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | more than 60% activity between pH 3.0 and 6.0 | Macrotyloma uniflorum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | the enzyme is stable between pH 4.0 and 6.5 | Macrotyloma uniflorum |