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Literature summary for 3.1.3.2 extracted from
- Biochemical characterization of Mycobacterium smegmatis RnhC (MSMEG_4305), a bifunctional enzyme composed of autonomous N-terminal type I RNase H and C-terminal acid phosphatase domains (2015), J. Bacteriol., 197, 2489-2498 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) RIPL cells | Mycolicibacterium smegmatis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D73N | the mutation effaces RNase H activity but has virtually no effect on the acid phosphatase activity of the RnhC protein | Mycolicibacterium smegmatis |
| H173A | the mutation does not affect RNase H activity but abolishes the acid phosphatase activity of the RnhC protein | Mycolicibacterium smegmatis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the acid phosphatase activity does not use a divalent cation cofactor | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His60-Ni Superflow resin column chromatography, Ni-NTA agarose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Mycolicibacterium smegmatis | 4-nitrophenol + phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rnhC | bifunctional enzyme with RNase H (N-terminus) and acid phosphatase (C-terminus) activities | Mycolicibacterium smegmatis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
4-nitrophenyl phosphate | at pH 5.5, temperature not specified in the publication | Mycolicibacterium smegmatis | |
| 0.33 | - |
4-nitrophenyl phosphate | at pH 4.5, temperature not specified in the publication | Mycolicibacterium smegmatis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Mycolicibacterium smegmatis |
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