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Literature summary for 3.1.3.2 extracted from

  • Wu, H.Y.; Liu, M.S.; Lin, T.P.; Cheng, Y.S.
    Structural and functional assays of AtTLP18.3 identify its novel acid phosphatase activity in thylakoid lumen (2011), Plant Physiol., 157, 1015-1025.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-tagged fusion protein Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
AtTLP18.3 proteins are crystallized by the hanging drop vapor diffusion method at 23°C. X-ray crystallography reveal the folding of AtTLP18.3 as a three-layer sandwich with 3 alpha-helices in the upper layer, 4 beta-sheets in the middle layer, and 2 alpha-helices in the lower layer, which resembles a Rossmann fold Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.89
-
 RRA(pT)VA synthetic phosphorylated oligopeptide, pH 4, 37°C Arabidopsis thaliana
12.49
-
 Ac-(pT)AILER synthetic phosphorylated oligopeptide, pH 4, 37°C Arabidopsis thaliana
12.56
-
 KR(pT)IRR synthetic phosphorylated oligopeptide, pH 4, 37°C Arabidopsis thaliana
13.88
-
 Ac-RK(pS)AGKPKN synthetic phosphorylated oligopeptide, pH 4, 37°C Arabidopsis thaliana
16.57
-
 Ac-(pT)IALGK synthetic phosphorylated oligopeptide, pH 4, 37°C Arabidopsis thaliana
42.56
-
 4-nitrophenyl phosphate pH 4, 37°C Arabidopsis thaliana
47.51
-
 O-phospho-L-serine pH 4, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity depends on divalent cations Arabidopsis thaliana
Fe2+ activity depends on divalent cations Arabidopsis thaliana
Mg2+ activity depends on divalent cations Arabidopsis thaliana
Mn2+ activity depends on divalent cations Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9ZVL6
-
-

Purification (Commentary)

Purification (Comment) Organism
using a GSTrap FF column Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
6,8 difluoro-4-methylumbelliferyl phosphate + H2O
-
 Arabidopsis thaliana 6,8 difluoro-4-methylumbelliferol + phosphate
-
 ?
Ac-(pT)AILER + H2O synthetic phosphorylated oligopeptide Arabidopsis thaliana ?
-
 ?
Ac-(pT)IALGK + H2O synthetic phosphorylated oligopeptide Arabidopsis thaliana ?
-
 ?
Ac-RK(pS)AGKPKN + H2O synthetic phosphorylated oligopeptide Arabidopsis thaliana ?
-
 ?
KR(pT)IRR + H2O synthetic phosphorylated oligopeptide Arabidopsis thaliana ?
-
 ?
O-phospho-L-serine + H2O
-
 Arabidopsis thaliana L-serine + phosphate
-
 ?
RRA(pT)VA + H2O synthetic phosphorylated oligopeptide Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
acid phosphatase
-
 Arabidopsis thaliana
AtTLP18.3
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 highest activity Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 assay at Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.5 highest activity Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana Northern blot analysis reveal AtTLP18.3 with a diurnally fluctuating expression pattern in wild-type plants. The transcripts show 2 high expression peaks, at 8 and 16 h after lights are 14 turned on up