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Literature summary for 3.1.3.2 extracted from
- Purification, primary structure, and properties of Euphorbia characias latex purple acid phosphatase (2011), Biochemistry, 76, 694-701.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Euphorbia characias |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| molybdate | inhibits enzymatic activity | Euphorbia characias |  |
| vanadate | inhibits enzymatic activity | Euphorbia characias | |
| Zn2+ | inhibits enzymatic activity | Euphorbia characias | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
4-nitrophenyl phosphate | pH 5.75, 25°C | Euphorbia characias | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | ELPAP contains 1 mol of Fe and 1 mole of Zn per mol of subunit | Euphorbia characias | |
| Zn | ELPAP contains 1 mol of Fe and 1 mole of Zn per mol of subunit | Euphorbia characias | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
SDS-PAGE | Euphorbia characias |
| 130000 | - |
native protein, gel filtration | Euphorbia characias |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euphorbia characias | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate fractionation, DEAE-cellulose chromatography and gel filtration chromatography | Euphorbia characias |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-bisphosphoglycerate + H2O | 85% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | ? | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Euphorbia characias | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | 100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | AMP + phosphate | - |
? | |
| AMP + H2O | 16% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | adenosine + phosphate | - |
? | |
| ATP + H2O | 100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | ADP + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | 25% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | 104% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | D-glucose + phosphate | - |
? | |
| GMP + H2O | 66% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | guanosine + phosphate | - |
? | |
| inositol 1-phosphate + H2O | 60% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | inositol + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | 96% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | pyruvate + phosphate | - |
? | |
| pyridoxal 5'-phosphate + H2O | 80% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | pyridoxal + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | each subunit contain one Fe(III) and one Zn(II) ion. The two subunits are connected by a disulfide bridge. 2 * 68000, SDS-PAGE | Euphorbia characias |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ELPAP | - |
Euphorbia characias |
| latex purple acid phosphatase | - |
Euphorbia characias |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
standard assay at | Euphorbia characias |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | enzyme is stable at 50°C but loses 70% of its activity at 60°C | Euphorbia characias |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 790 | - |
4-nitrophenyl phosphate | pH 5.75, 25°C | Euphorbia characias | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.75 | - |
- |
Euphorbia characias |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
vanadate | pH 5.75, 25°C | Euphorbia characias | |
| 0.0008 | - |
molybdate | pH 5.75, 25°C | Euphorbia characias | |
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