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Literature summary for 3.1.3.2 extracted from

  • Hoopman, T.C.; Wang, W.; Brautigam, C.A.; Sedillo, J.L.; Reilly, T.J.; Hansen, E.J.
    Moraxella catarrhalis synthesizes an autotransporter that is an acid phosphatase (2008), J. Bacteriol., 190, 1459-1472.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Moraxella catarrhalis
expressed in Escherichia coli DH5alpha Moraxella catarrhalis

Protein Variants

Protein Variants Comment Organism
H110A mutant protein shows enzyme activity Moraxella catarrhalis
H233A site-directed mutagenesis of a critical histidine residue (H233A) in the predicted active site of the acid phosphatase domain in MapA eliminates acid phosphatase activity in the recombinant MapA protein Moraxella catarrhalis
H233A active site residue, no enzyme activity Moraxella catarrhalis
additional information inactivation of the mapA gene reduces the acid phosphatase activity expressed by this organism, and this mutation could be complemented in trans with the wild-type mapA gene Moraxella catarrhalis
additional information the central region of the MapA protein shows similarity to passenger domains of other autotransporter proteins, whereas the C-terminal portion of MapA resembles the translocation domain of conventional autotransporters. Use of the predicted MapA translocation domain in a fusion construct with the passenger domain from another predicted Moraxella catarrhalis autotransporter confirms the translocation ability of this MapA domain Moraxella catarrhalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the MapA protein is shown to be localized to the outer membrane and is not detected either in the soluble cytoplasmic fraction from disrupted Moraxella catarrhalis cells or in the spent culture supernatant fluid from Moraxella catarrhalis Moraxella catarrhalis 16020
-
outer membrane
-
Moraxella catarrhalis 19867
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
calculated from cDNA Moraxella catarrhalis

Organism

Organism UniProt Comment Textmining
Moraxella catarrhalis A9XDF5
-
-
Moraxella catarrhalis A9XED1
-
-
Moraxella catarrhalis A9XED2
-
-
Moraxella catarrhalis A9XED3
-
-
Moraxella catarrhalis A9XED4
-
-
Moraxella catarrhalis 7169 A9XED2
-
-
Moraxella catarrhalis ETSU-9 A9XED3
-
-
Moraxella catarrhalis O12E.44 A9XED1
-
-
Moraxella catarrhalis O35E A9XDF5
-
-
Moraxella catarrhalis V1120 A9XED4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Moraxella catarrhalis 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Moraxella catarrhalis O35E 4-nitrophenol + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Moraxella catarrhalis p-nitrophenol + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Moraxella catarrhalis O35E p-nitrophenol + phosphate
-
?
phenolphthalein diphosphate + H2O
-
Moraxella catarrhalis ? + phosphate
-
?
phenolphthalein diphosphate + H2O
-
Moraxella catarrhalis O35E ? + phosphate
-
?

Synonyms

Synonyms Comment Organism
acid phosphatase A
-
Moraxella catarrhalis
acid phosphatase MapA autotransporter with acid phosphatase activity Moraxella catarrhalis
mAPA
-
Moraxella catarrhalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Moraxella catarrhalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Moraxella catarrhalis