Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Moraxella catarrhalis |
expressed in Escherichia coli DH5alpha | Moraxella catarrhalis |
Protein Variants | Comment | Organism |
---|---|---|
H110A | mutant protein shows enzyme activity | Moraxella catarrhalis |
H233A | site-directed mutagenesis of a critical histidine residue (H233A) in the predicted active site of the acid phosphatase domain in MapA eliminates acid phosphatase activity in the recombinant MapA protein | Moraxella catarrhalis |
H233A | active site residue, no enzyme activity | Moraxella catarrhalis |
additional information | inactivation of the mapA gene reduces the acid phosphatase activity expressed by this organism, and this mutation could be complemented in trans with the wild-type mapA gene | Moraxella catarrhalis |
additional information | the central region of the MapA protein shows similarity to passenger domains of other autotransporter proteins, whereas the C-terminal portion of MapA resembles the translocation domain of conventional autotransporters. Use of the predicted MapA translocation domain in a fusion construct with the passenger domain from another predicted Moraxella catarrhalis autotransporter confirms the translocation ability of this MapA domain | Moraxella catarrhalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the MapA protein is shown to be localized to the outer membrane and is not detected either in the soluble cytoplasmic fraction from disrupted Moraxella catarrhalis cells or in the spent culture supernatant fluid from Moraxella catarrhalis | Moraxella catarrhalis | 16020 | - |
outer membrane | - |
Moraxella catarrhalis | 19867 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103000 | - |
calculated from cDNA | Moraxella catarrhalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moraxella catarrhalis | A9XDF5 | - |
- |
Moraxella catarrhalis | A9XED1 | - |
- |
Moraxella catarrhalis | A9XED2 | - |
- |
Moraxella catarrhalis | A9XED3 | - |
- |
Moraxella catarrhalis | A9XED4 | - |
- |
Moraxella catarrhalis 7169 | A9XED2 | - |
- |
Moraxella catarrhalis ETSU-9 | A9XED3 | - |
- |
Moraxella catarrhalis O12E.44 | A9XED1 | - |
- |
Moraxella catarrhalis O35E | A9XDF5 | - |
- |
Moraxella catarrhalis V1120 | A9XED4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Moraxella catarrhalis | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Moraxella catarrhalis O35E | 4-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Moraxella catarrhalis | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Moraxella catarrhalis O35E | p-nitrophenol + phosphate | - |
? | |
phenolphthalein diphosphate + H2O | - |
Moraxella catarrhalis | ? + phosphate | - |
? | |
phenolphthalein diphosphate + H2O | - |
Moraxella catarrhalis O35E | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid phosphatase A | - |
Moraxella catarrhalis |
acid phosphatase MapA | autotransporter with acid phosphatase activity | Moraxella catarrhalis |
mAPA | - |
Moraxella catarrhalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Moraxella catarrhalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Moraxella catarrhalis |